Which of the Following Is NOT True for HPLC Sample

Which of the following is NOT true for HPLC sample injection.

A) Reproducibly load injector loop with < 0.5 or > 3 loop volumes depending on injection technique.
B) The injection loops must be removed and cleaned after each injection.
C) Sample solvent strength should be less than or equal to eluent solvent strength.
D) Pass samples through a 0.5-m filter to remove particulate matter prior to injection.
E) Use a blunt HPLC syringe needle,not pointed,to prevent damage to the injection port.

Correct Answer:

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