What Supporting Evidence Can Be Used to Decide Whether Variation

What supporting evidence can be used to decide whether variation in a gene is actually a mutation?

A) It is never found in people without the phenotype of interest
B) It must be in an exon
C) Additional variation is found in the same gene in other people with a similar phenotype
D) It is expressed only in a tissue relevant to the phenotype
E) All of the above

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