Question 1

Which of the following terms is for the specific sequences in DNA that determine intrinsic RNA chain cessation?

Accepted Answer

A)  termination
B)  rho binding
C)  template binding
D)  supercoil A

Question 2

Which of the following base locations in DNA specifies the first base in the RNA transcript designated,and what numerical value is it assigned?

Accepted Answer

A)  promoter;-5
B)  enhancer;-35
C)  transcription start site;0
D)  transcription start site;+1 D

Question 3

Which of the following a-amanitin-resistant RNA polymerases carries out transcription in eukaryotic cells?

Accepted Answer

A)  I only
B)  II only
C)  III only
D)  I and III A

Question 4

Which of the following is a feature of transcription in eukaryotic cells?

Accepted Answer

A)  Mature transcripts undergo continuous processing.
B)  Transcription and translation are spatially coupled.
C)  mRNAs are polycistronic.
D)  Transcription occurs in the nucleus.

Question 5

Which of the following terms defines the expression of genes independently of regulation?

Accepted Answer

A)  autoregulation
B)  constitutive expression
C)  transcription expression
D)  repressible regulation

Question 6

Which of the following may NOT be a constituent of RNA polymerase II promoters?

Accepted Answer

A)  the core element where general transcription factors bind
B)  the site where enhancers bind transcriptional activators
C)  the site where silencers bind inducers
D)  a TATA box indicating the transcription start site

Question 7

Which of the following processes is the initial event in the conversion of an hnRNA to the mature RNA?

Accepted Answer

A)  formation of ribonucleoprotein particles (RNPs) 
B)  capping the 5'-end of the transcript with a guanylyl group by guanylyl transferase
C)  addition of a poly(
A)  tail to the 3'-end of the transcript by poly(
A)  polymerase
D)  transesterification reactions in lariat formation

Question 8

Which of the following terms for gene expression is often responsive to small molecules that increase synthesis of enzymes for metabolism of a certain substrate?

Accepted Answer

A)  autoregulation
B)  co-induction
C)  induction
D)  repression

Question 9

Operons are regulatory sequences lying adjacent to the DNA being transcribed and are composed of a promoter located next to an operator.Which of the following interacts with and controls the operator?

Accepted Answer

A)  repression protein
B)  enhancer protein
C)  activator protein
D)  regulatory protein

Question 10

Which of the following is a property of the lac operon?

Accepted Answer

A)  Allolactose increases the effect of the repressor.
B)  Lactose represses the lac operon.
C)  It is negatively controlled via CAP.
D)  It is negatively controlled via the lac repressor.

Question 11

Which of the following factors is an ATP-dependent helicase utilized in one of the termination mechanisms in bacteria?

Accepted Answer

A)  α (alpha) 
B)  β (beta) 
C)  ρ (rho) 
D)  δ (delta)

Question 12

Which of the following is a characteristic of catabolic activator protein (CAP)?

Accepted Answer

A)  ensures that the operons necessary for metabolism for alternate energy sources remain activated until the supply of glucose is exhausted
B)  C-terminal end binds cAMP,and N-terminal end binds DNA
C)  overrides the influence of any inducer that might be present
D)  N-terminal end binds cAMP,and C-terminal end binds DNA

Question 13

Which of the following is a feature of transcription activation?

Accepted Answer

A)  A nucleotide sequence binding site for a DNA-binding protein serves as an activator site if DNA-binding protein can interact with promoter-bound RNA polymerase.
B)  It takes place when a transcriptional activator protein bound to DNA makes protein:RNA contacts with RNA polymerase.
C)  The degree of transcriptional activation is inversely proportional to the strength of the protein:protein interaction.
D)  If the DNA-bound transcriptional activator makes contact with 2 different components of RNA polymerase,the transcription is markedly reduced.

Question 14

Which of the following is a characteristic of homeobox domains?

Accepted Answer

A)  act as sequence-specific transcriptional factors
B)  typically comprise about 90% of the protein mass
C)  found in only a few eukaryotes
D)  contain helix-b-strand-helix motifs

Question 15

Which of the following is NOT a characteristic of intron excision and exon ligation (splicing)?

Accepted Answer

A)  It occurs exclusively in the endoplasmic reticulum.
B)  It involves hnRNA in ribonucleoprotein particles.
C)  hnRNA is capped and polyadenylated.
D)  It relies on snRNPs.

Question 16

Which of the following occurs when the cellular level of tryptophan decreases in E.coli?

Accepted Answer

A)  Tryptophan-bound Trp repressor associates with trp operator.
B)  Trp apo-repressor has lowered affinity for trp promoter,allowing RNA polymerase binding and transcription of the trp operon.
C)  Tryptophan binds trp inducer to promote positive control of trp promoter.
D)  Trp repressor has a greater affinity for trp operator.

Question 17

Which of the following is NOT a part of the process that leads to the production of multiple transcripts from a single gene?

Accepted Answer

A)  constitutive splicing
B)  use of different promoters
C)  selection of different polyadenylation sites
D)  alternative splicing of the primary transcript

Question 18

Which of the following processes is used by both prokaryotes and differentiated eukaryotic cells when they express a small number of their genes at any given time?

Accepted Answer

A)  transcription
B)  translation
C)  replication
D)  repair

Question 19

Which of the following a feature of TATA boxes?

Accepted Answer

A)  They are found outside the core sequence of a gene regulatory sequence.
B)  They are found downstream of the gene being transcribed.
C)  They reside in the enhancer regions.
D)  They represent an area of DNA more easily opened than other areas.

Question 20

Which of the following subunits in E.coli RNA polymerase is required for recognition of the promoter sequence?

Accepted Answer

A)  α (alpha) 
B)  β (beta) 
C)  σ (sigma) 
D)  β′ (beta prime)

Exam 29: Transcription and the Regulation of Gene Expression