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When Using Cassette Mutagenesis, in Order to Insert an Engineered

Question 17

Multiple Choice

When using cassette mutagenesis, in order to insert an engineered cDNA sequence into a plasmid, a __________ is used to cut out the sequence that is no longer needed, and a __________ is used to insert the new mutant cassette into the plasmid.


A) restriction enzyme; ligase
B) clone; enzyme
C) catalase; polymerase
D) reductase; polymerase
E) all of the above

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