Using Site-Directed Mutagenesis, the Mutation of a Serine Residue to an Alanine

Using site-directed mutagenesis, the mutation of a serine residue to an alanine residue might be used to _________.
I. decrease the solubility of a protein
II. eliminate the possibility of phosphorylation at that location
III. observe the impact of a non-polar side chain at that location
IV. prove that serine had a catalytic function in the native protein
V. promote proper folding of the protein

A) I and II
B) I and III
C) II, III and IV
D) II, IV and V
E) I, II and V

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