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How Does Reverse-Phase Chromatography Differ from HIC

Question 34

Multiple Choice

How does reverse-phase chromatography differ from HIC?


A) In HIC, eluting buffers can be gentler
B) In HIC, the hydrophobic interactions between proteins and resin are weaker
C) In HIC, the hydrophobic interactions between proteins and resin are stronger
D) In reverse-phase, low MW proteins elute from the column earliest
E) Both A and B

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