Multiple Choice
High pressure liquid chromatography (HPLC) is not best for separating proteins because
A) it takes too long for high molecular weight molecules to elute
B) only highly acidic buffers can be used which alter proteins' primary structures
C) its required metal tubing interferes with protein purification
D) fast protein liquid chromatography (FPLC) runs at higher and more useful pressures
E) it cannot be accomplished in the cold
Correct Answer:
Verified
Correct Answer:
Verified
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