Question 1

Weigert's stain is an example of what stain?

Accepted Answer

A) Copper hematoxylin 
B) Iron hematoxylin 
C) Alum hematoxylin 
D) Ripened hematoxylin

Question 2

The process of ripening can be accelerated through these oxidizing agents. What are these agents?

Accepted Answer

A) Hydrogen peroxide 
B) Mercuric oxide 
C) Potassium permanganate 
D) All of the above

Question 3

Best Carmine solution is a combination of Carmine and what substance?

Accepted Answer

A) Picric Acid 
B) Ammonia 
C) Aluminum Chloride 
D) Mercuric chloride

Question 4

Which of the following vital stains is said to be dangerous and becomes toxic to the cells if the suspension stands for more than one hour?

Accepted Answer

A) Neutral red 
B) Janus green 
C) Trypan blue 
D) Nile Blue

Question 5

This oil soluble dye has no secondary amino group, making it hard to stain phospholipids. What is this dye?

Accepted Answer

A) Sudan Black 
B) Sudan III 
C) Sudan IV 
D) Oil Red O

Question 6

This staining method is use in Reticulocyte counting. What do you call this method?

Accepted Answer

A) Supravital staining 
B) Direct staining 
C) Intravital staining 
D) None of the above

Question 7

Which among the following methods can the Schiff reagent be possibly prepared?

Accepted Answer

A) Barger and Delamater 
B) De Tomasi Coleman 
C) Hikawa and Oguru 
D) All of the above

Question 8

In the indirect staining method, this non-mordant substance is not essential to the chemical union of tissue and dye and does not participate but only accelerates or hastens the speed of the staining reaction.

Accepted Answer

A) Accentuator 
B) Dye 
C) Mordant 
D) Accelerator

Question 9

This is the process of staining whereby the tissues are overstained to obligate the cellular details and the excess stain is removed or decolorized from unwanted parts of the tissue.

Accepted Answer

A) Progressive staining 
B) Regressive staining 
C) Indirect staining 
D) Direct staining

Question 10

It is a basic dye recommended for observing cell granules and vacuoles of phagocytic cells?

Accepted Answer

A) Neutral red 
B) Night blue 
C) Orcein 
D) Methylene violet

Question 11

This is a fluorescent red dye commonly used as a background stain particularly in chromate and picric acid fixed tissues and in acid decalcified materials?

Accepted Answer

A) Hematoxylin 
B) Eosin 
C) Methylene blue 
D) Iodine

Question 12

What is the best tissue section when staining with Best Carmine Method?

Accepted Answer

A) Paraffin section 
B) Celloidin section 
C) Frozen section 
D) Cryostat section

Question 13

In preparing the Schiff reagent through the Barger and Delamater method, what solution is added to the Schiff reagent?

Accepted Answer

A) Thionyl chloride 
B) Sulfur dioxide gas 
C) Sodium potassium metabisulfite 
D) None of the above

Question 14

What iron hematoxylin solution uses ferric ammonium sulfate as a mordant?

Accepted Answer

A) Weigert's 
B) Heidenhain's 
C) PTAH 
D) All of the above

Question 15

What stain is used as a counterstain in Best Carmine Method?

Accepted Answer

A) Cole's hematoxylin 
B) Harris' hematoxylin 
C) Ehrlich's hematoxylin 
D) Mayer's hematoxylin

Question 16

Lindquist's Modified Rhodanine Technique is used for staining Copper. In this technique, what medium is preferred for mounting?

Accepted Answer

A) Farrant's medium 
B) Brun's fluid 
C) Apathy's medium 
D) Glycerin

Question 17

When sections are stained with simple Aqueous or Alcoholic solutions of the dye, what you call this staining process?

Accepted Answer

A) Progressive staining 
B) Regressive staining 
C) Direct staining 
D) Indirect staining

Question 18

The addition of this substance will help improve the ability of the carmine solution to stain for mucin. What is this substance?

Accepted Answer

A) Aluminum chloride 
B) Aluminum hydroxide 
C) Potassium chloride 
D) Ammonium chloride

Question 19

In glycogen demonstration, what stain is widely used?

Accepted Answer

A) Methylene blue 
B) Aniline blue 
C) Carmine 
D) Periodic Acid Schiff

Question 20

Supravital stains are dyes used to stain living cells after removal from the living body. What is the best vital stain?

Accepted Answer

A) Neutral red 
B) Janus green 
C) Nile blue 
D) Toluidine blue

Exam 5: Staining