Passage Adeno-Associated Virus 2 (AAV2) Is a Nonpathogenic, Nonenveloped DNA Virus

Passage
Adeno-associated virus 2 (AAV2) is a nonpathogenic, nonenveloped DNA virus that requires helper viruses such as adenovirus (AV) or herpes simplex virus (HSV) to replicate efficiently.  Because it can transduce human cells, including quiescent (nondividing) cells, without causing disease, AAV2 is attractive as a gene therapy vector.  In clinical trials, a modified AAV2 virus has successfully treated the retinal disease Leber's congenital amaurosis as well as the blood clotting disorder hemophilia B.  AAV2 transduces cells via a multistep process, shown in Figure 1.
Figure 1  Schematic showing the mechanism of transduction by AAV2Studies indicate that unlike AV and HSV, AAV2 inhibits pathways other than the endosomal pathway, such as trafficking to the Golgi, increasing the percentage of invading virus particles that arrive at the nucleus.
Adapted from Xiao PJ, Samulski RJ. Cytoplasmic trafficking, endosomal escape, and perinuclear accumulation of adeno-associated virus type 2 particles are facilitated by microtubule network. J Virol. 2012;86(19) :10462-73.
-AAV2 entry into cells could best be blocked by a drug that inhibits which of the following mechanisms?

A) Fusion of viral and cell membranes upon receptor binding
B) Formation of membrane extensions that engulf external particles
C) Inward budding of the cell in response to receptor binding
D) Constitutive invagination of the cell membrane to take up extracellular liquid

Correct Answer:

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