As Part of a Research Study Investigating Enzymatic Activity in Both

As part of a research study investigating enzymatic activity in both normal and diseased liver tissue, hepatocytes are isolated from biopsy samples obtained from patients undergoing routine care at a local tertiary referral center.  The cells are homogenized and centrifuged to remove membrane components and organelles.  Following subsequent rounds of centrifugation, the remaining supernatant contains only cytosol and cytosolic proteins.  Activity of which of the following enzymes will most likely be detectable in the supernatant of healthy liver cells?

A) 3-Hydroxy-3-methylglutaryl-CoA lyase
B) Ornithine transcarbamylase
C) Pyruvate carboxylase
D) Succinate dehydrogenase
E) Transketolase

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