Question 1

What are transgenic organisms? Explain the rationale behind using these organisms to produce some of the proteins needed for human use, instead of relying solely on proteins produced by genetically engineered E. coli.

Accepted Answer

The answer of What are transgenic organisms? Explain the rationale...

Question 2

Scientists study animal models in order to reveal the functions of human genes. This is an example of:

Accepted Answer

A)  comparative genomics 
B)  DNA cloning 
C)  Western blotting 
D)  RNAi 
E)  gene targeting

Question 3

Cosmid cloning vectors accommodate much larger fragments of DNA than bacterial artificial chromosomes.

Accepted Answer

A) True 
 B)False

Question 4

cDNA libraries contain:

Accepted Answer

A)  single-stranded DNA 
B)  DNA synthesized with the use of DNA polymerase 
C)  DNA synthesized from and complementary to an mRNA template 
D)  introns and exons 
E)  genomic DNA

Question 5

Match between columns
                        Premises: Step 3

Accepted Answer

The Answer of The blotting buffer transfers DNA fragments to is...

Question 6

Match between columns
                        Premises: Transgenic salmon that grow faster than wild salmon may result in less pollution from salmon farms.

Accepted Answer

The Answer of Disadvantage and Advantage is...

Question 7

What is meant by a genetic shotgun?

Accepted Answer

The answer of What is meant by a genetic shotgun?...

Question 8

A genomic library consists of fragments of all DNA:

Accepted Answer

A)  that occurs in one species 
B)  contained in one chromosome 
C)  that would be found in a typical cell of an organism 
D)  in a recombinant gene 
E)  that codes for functional enzymes in a typical cell of an organism

Question 9

"Bt corn" are corn plants that contain a bacterial gene for the bacterial toxin, Bt. The production of this toxin kills insects that feed on the plant, thereby making the plants pest-resistant. This process is an example of:

Accepted Answer

A)  chemical engineering 
B)  genomic studies 
C)  fluorescent GloFish 
D)  recombinant DNA technology 
E)  genetic engineering

Question 10

Match between columns
                        Premises: Transgenic pest-resistant crops may kill all vulnerable individuals, creating a population of pesticide-resistant pests.

Accepted Answer

The Answer of Disadvantage and Advantage is...

Question 11

PCR has many uses and advantages. What is one disadvantage of this technology?

Accepted Answer

A)  It is difficult to keep the amplified DNA strands intact. 
B)  PCR works on only some DNA sequences. 
C)  The amplified product is often degraded. 
D)  PCR is a slow and inefficient process. 
E)  PCR is easily contaminated with foreign DNA.

Question 12

The polymerase chain reaction was developed by:

Accepted Answer

A)  Francis Crick 
B)  Walter Gilbert 
C)  E.M. Southern 
D)  Kary Mullis 
E)  Leland Hartwell

Question 13

Which of the following statements characterizes PCR?

Accepted Answer

A)  PCR can be performed with any DNA polymerase. 
B)  PCR is only useful for amplifying large quantities of DNA in vitro . 
C)  PCR replicates specific DNA sequences. 
D)  PCR has not been successful for archaeological and crime scene analyses. 
E)  PCR utilizes Taq polymerase, which remains active only at low temperatures.

Question 14

Match between columns
                        Premises: Step 4

Accepted Answer

The Answer of The radiograph shows the hybridized DNA fragments. is...

Question 15

Plasmid cloning vectors can:

Accepted Answer

A)  accommodate DNA fragments up to 23 kb 
B)  transport a DNA fragment from the nucleus to the cytoplasm 
C)  accommodate DNA fragments up to 10 kb 
D)  accommodate fragments up to 350 kb 
E)  infect and insert their DNA into bacterial cells

Question 16

A large number of copies of any DNA segment can be obtained by:

Accepted Answer

A)  introducing foreign DNA into a microorganism so that it can be replicated 
B)  stimulating increased transcription of the appropriate sequence of mRNA 
C)  stimulating increased translation of the appropriate DNA molecule 
D)  inducing the production of DNA from proteins 
E)  stimulating reverse transcription of tRNA

Question 17

In the field of plant transgenics, the use of the bacterium Agrobacterium tumefaciens has proven useful because the bacterium:

Accepted Answer

A)  is not attacked by plant immune systems 
B)  has been genetically engineered to penetrate the plant cell wall 
C)  can directly modify the plant genome 
D)  is found naturally in almost all species of plants 
E)  is able to transfer a plasmid into host plant cells

Question 18

Why is it unlikely that genetically engineered strains of bacteria would pose a threat to the environment?

Accepted Answer

A)  Genetically engineered bacteria cannot survive exposure to UV radiation. 
B)  Genetically engineered strains of bacteria are strictly regulated, making accidental release unlikely. 
C)  Genetically engineered strains of bacteria cannot survive exposure to oxygen. 
D)  Genetically engineered strains of bacteria are poor competitors of wild strains. 
E)  Genetically engineered strains of bacteria require supplemental nutrients to survive.

Question 19

A cDNA library is complementary to DNA and thus does not contain introns.

Accepted Answer