Subjects in a Multiple Group Experimental Study Are Tested for How

Subjects in a multiple group experimental study are tested for how much time it takes them to navigate a maze and find the chocolate. The maze is reconstructed after each run, and three different floor plans are used. Each group is tested eight times in 8 hours at a different time of day. The runs later in the day have faster times than the earlier ones. Which threat to internal validity might account for this difference?

A) Instrumentation
B) Selection
C) Testing
D) Statistical regression toward the mean

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