Question 1

All of the following can be true of cloning vectors EXCEPT that they

Accepted Answer

A)  are plasmids that slip into a host cell.
B)  are plasmids that accept foreign DNA.
C)  can be engineered to contain multiple cloning sites.
D)  are portions of the main bacterial chromosome.
E)  can be viruses. D

Question 2

In human gene therapy, genes are transferred by

Accepted Answer

A)  viral vectors or lipid clusters
B)  bacterial cells.
C)  bacterial plasmids.
D)  hypodermic injection.
E)  tissue transplantation. A

Question 3

Even though nature has been carrying out genetic experiments for 3.8 billion years, what may be the unsettling aspect of biologists' foray into this endeavor is

Accepted Answer

A)  that humans are now "playing god."
B)  uncertainty about our ability to evaluate the impact
C)  that scientists really do not know what they are doing in this area.
D)  the number of dangerous plants and animals that have been produced.
E)  the new diseases that could be created. B

Question 4

The value of inserting human genes suspected of causing Alzheimer disease into mice is to

Accepted Answer

A)  make mice act more human.
B)  make antibodies, which can be injected into suffering patients.
C)  develop mice that can serve as models for study of this condition.
D)  use the mice for production of a vaccine for the disease.
E)  accomplish all of these.

Question 5

Enzymes used to cut genes in recombinant DNA research are

Accepted Answer

A)  ligases.
B)  restriction enzymes.
C)  transcriptases.
D)  DNA polymerases.
E)  replicases.

Question 6

The animals most likely to be used for xenotransplantation of organs into humans are

Accepted Answer

A)  goats.
B)  sheep.
C)  cattle.
D)  pigs.
E)  dogs.

Question 7

For polymerase chain reaction to occur,

Accepted Answer

A)   primers must hydrogen-bond to the template DNA
B)  all DNA fragments must be identical. 
C)   the DNA must remain double stranded.
D)  a sticky end must be available for the ligase enzyme to function.
E)  all of these must occur.

Question 8

A collection of host cells that houses different cloned fragments of DNA is a

Accepted Answer

A)  cDNA library.
B)  transcribed DNA library.
C)  genomic library.
D)  DNA library.
E)  plasmid library.

Question 9

Human insulin is produced in transgenic

Accepted Answer

A)  bacteria.
B)  viruses.
C)  fungi.
D)  plants.
E)  animals.

Question 10

Nucleic acid hybridization is the pairing between

Accepted Answer

A)  cDNA and DNA.
B)  mRNA and cDNA.
C)  mRNA and DNA.
D)  mRNA and tRNA.
E)  DNA or RNA from more than one source.

Question 11

Among the effects of vitamin A deficiency are

Accepted Answer

A)  dry skin, eyes, and mucous membranes.
B)  increased vulnerability to infections of the skin, eyes, and mucous membranes.
C)  impaired growth and development.
D)  mental impairment.
E)  all of these.

Question 12

The process illustrated in the figure is a step in

Accepted Answer

A)  the production of recombinant DNA. 
B)  probing for transferred genes.
C)  DNA fingerprinting.
D)  sequencing DNA.
E)  producing radioactive probes.

Question 13

Cross-breeding produced featherless chickens for which of the following purposes?

Accepted Answer

A)  to make it easier to process after slaughter
B)  to prevent a feather infecting disease
C)  to be more resistant to high temperatures in the desert
D)  to improve breeding efficiency
E)  to do all of these

Question 14

cDNA

Accepted Answer

A)  does not contain introns.
B)  is produced from mRNA.
C)  production utilizes reverse transcriptase.
D)  begins as a hybrid molecule with an mRNA.
E)  fits all of these descriptions.

Question 15

Gel electrophoresis separates the DNA fragments according to

Accepted Answer

A)  their length.
B)  their mass.
C)  their speed of travel through the gel.
D)  the number of nucleotides in the fragment.
E)  all of these.

Question 16

Restriction enzymes

Accepted Answer

A)  work at specific base sequences
B)  function only at "sticky ends."
C)  produce uniform lengths of DNA.
D)  function in viruses.
E)  are produced by viruses to break down bacterial DNA.

Question 17

Cloned fragments of all of the DNA in a haploid number of chromosomes characterizing a species is a

Accepted Answer

A)  cDNA library.
B)  transcribed DNA library.
C)  genomic library.
D)  gene library.
E)  plasmid library.

Question 18

Thermus aquaticus was important in the development of PCR in that it provided the necessary

Accepted Answer

A)  DNA polymerase. 
B)   reverse transcriptase.
C)  primers.
D)  DNA template.
E)  DNA ligase.

Question 19

Researchers can deliver genes into plants by using

Accepted Answer

A)  the bacterium Agrobacterium tumefaciens.
B)  microscopic particles coated with DNA.
C)  electric shocks.
D)  chemicals.
E)  all of these.

Question 20

In order for DNA molecules to undergo recombination,

Accepted Answer

A)  they must be from the same species.
B)  their strands must separate as in replication.
C)  they must be cut and spliced at specific nucleotide sequences.
D)  one of the two DNA strands must be degraded.
E)  they must first be transcribed.

Exam 16: Recombinant Dna and Genetic Engineering