Question 1

What does MALDI stand for?

Accepted Answer

A)  Maser- Assisted Ligand Desorption Ionization
B)  Maser- Assisted Laser Desorption Ionization
C)  Matrix- Assisted Ligand Desorption Ionization
D)  Matrix- Assisted Laser Desorption Ionization D

Question 2

A common first separation technique in 2D gels is isoelectric focusing.What property is isoelectric focusing taking advantage of?

Accepted Answer

A)  charge
B)  amino acid content
C)  the pH at which the overall charge is zero
D)  size C

Question 3

A newly sequenced gene is cloned and expressed in a bacterial cell.The bioinformatics databases suggest that this gene produces an enzyme that catalyzes the conversion of carbon dioxide to bicarbonate.After weeks of experiments,a scientist determined the enzyme has a completely different function.This is an example of_______ .

Accepted Answer

A)  incorrect qPCR
B)  incorrect BLAST parameters
C)  incorrect annotation
D)  incorrect enzyme experiments
E)  incorrect sequencing C

Question 4

Numerous scientists around the world have proposed to sequence 10,000 vertebrate genomes in five years.What is the name of this plan?

Accepted Answer

A)  10K or Bust
B)  Vertebrate Beginnings
C)  Bigger Than Life Plan
D)  Genome 10K
E)  Vertebrate Enlightenment

Question 5

A drawback to using microarrays is_______ .

Accepted Answer

A)  the spots are very tiny
B)  the arrays do not cover enough of the genome
C)  researcher- made arrays have high variability
D)  commercial microarrays have high variability in quality

Question 6

Personal genome sequencing assists with the recognition and determination of what?

Accepted Answer

A)  somatic genome mosaicism
B)  locations for introns
C)  inheritable diseases and their alleles
D)  silent mutations in the genome

Question 7

DNA microarray analysis is excellent for _______.

Accepted Answer

A)  studying one gene's expression very closely
B)  studying all of a sample's expressed genes simultaneously
C)  studying all of a sample's unexpressed genes simultaneously
D)  studying all of a sample's genes simultaneously

Question 8

Several years ago,scientists compared the genome of a wooly mammoth to that of an African elephant and found them to be 98.5% identical.This is an example of what type of "- omics"?

Accepted Answer

A)  stone- age genomics
B)  space- age genomics
C)  exomics
D)  proteomics
E)  extinctomics

Question 9

Genomes from several primates have been sequenced and annotated.These primates range from chimpanzee to Rhesus monkey to Neanderthal and modern humans.What is the advantage of having so many primate sequences available to researchers?

Accepted Answer

A)  Comparative genomics allows for determining evolutionary trends.
B)  The primate sequences can be compared to learn about dietary trends.
C)  Phenotypes can be determined from the genomes.
D)  The primate sequences can be compared to learn about social behavior.

Question 10

A microbiome is essential for human infant development.Where does an infant obtain the origins of its microbiome?

Accepted Answer

A)  the infant has a microbiome at conception
B)  the hospital
C)  the father
D)  the mother

Question 11

Most of the bacterial genomes described in the text have fewer than_______ .

Accepted Answer

A)  10,000 genes
B)  500 genes
C)  50 genes
D)  5000 base pairs
E)  10,000 base pairs

Question 12

Nutrigenomics is the study of_______ .

Accepted Answer

A)  the vitamins and minerals in the genome
B)  the effect of genomics on dietary desires
C)  the relationship between diet and the genome
D)  the effect of nutrition on genome sequences

Question 13

In general,the organization of genes in bacteria is different from that in eukaryotes.In E.coli,approximately 27 percent of all genes are organized into contiguous,functionally related units containing multiple genes under coordinated control that are transcribed as a single unit.Such contiguous gene families are called_______ .

Accepted Answer

A)  proteomes
B)  operons
C)  pseudogenes
D)  transcriptomes
E)  contigs

Question 14

Compared with eukaryotic chromosomes,bacterial chromosomes are_______ .

Accepted Answer

A)  large,mainly organized in single- gene transcription units without introns
B)  small,mainly organized in single- gene transcription units with introns
C)  large,mainly organized in polygenic transcription units without introns
D)  large,triple- helix,Z- DNA,organized in single gene units with introns
E)  small,with high gene density

Question 15

What is one cause for the 21,000 protein encoding sequences in the human genome producing between 200,000 and 1 million different proteins?

Accepted Answer

A)  CNVs
B)  CRISPR- Cas systems
C)  restriction enzymes
D)  alternative splicing
E)  SNPs

Question 16

Transcriptomics is _______.

Accepted Answer

A)  measuring how much DNA is in the genome
B)  the quantification of gene expression
C)  determining phenotype
D)  determining epigenetic markers

Question 17

The genome of the domesticated dog,Canis familiaris,was sequenced in 2005.Using the dog as a model system provides insight into which of the following human disease states?

Accepted Answer

A)  aneuploidies,multifactorial diseases,and behavioral conditions
B)  aneuploidies
C)  multifactorial diseases
D)  behavioral conditions i.e.,obsessive- compulsive disorders)

Question 18

Using RNA- seq generates data in situ.What does RNA- seq not provide data for?

Accepted Answer

A)  sequence of the RNA
B)  location of transcript in the genome
C)  amount of protein produced by RNA
D)  gene expression levels

Question 19

One major difference between prokaryotic and eukaryotic genes is that eukaryotic genes can contain internal sequences,called_______ ,which get removed in the mature message.

Accepted Answer

A)  inteins
B)  exons
C)  exteins
D)  splice- outs
E)  introns

Question 20

Transcriptomics requires the isolation of RNA to generate sequence data.If a scientist wants to use next- generation sequencing to perform their experiment which of the following is necessary?

Accepted Answer

A)  protein quantification
B)  euchromatin
C)  reverse transcriptase
D)  heterochromatin

Exam 21: Genomic Analysis