Question 1

One of the most common organisms used to produce knockouts is ________.

Accepted Answer

A)  cows 
B)  horses 
C)  mice 
D)  Drosophila 
E)  All of the above 
A)  cows 
B)  horses 
C)  mice 
D)  Drosophila 
E)  All of the above C

Question 2

________ animals contain an inserted and functional gene from another source.

Accepted Answer

A)  Knockout 
B)  Transgenic 
C)  Polyploid 
D)  Selectively bred 
E)  Cloned 
A)  Knockout 
B)  Transgenic 
C)  Polyploid 
D)  Selectively bred 
E)  Cloned B

Question 3

________ animals have been genetically engineered so that a specific gene is disrupted and rendered nonfunctional.

Accepted Answer

A)  Knockout 
B)  Transgenic 
C)  Polyploid 
D)  Selectively bred 
E)  Cloned 
A)  Knockout 
B)  Transgenic 
C)  Polyploid 
D)  Selectively bred 
E)  Cloned A

Question 4

What are the three "Rs" related to regulation of animal research?

Accepted Answer

The three Rs are:
• reduce the number of

Question 5

Homologous recombination ________.

Accepted Answer

A)  occurs during meiosis as a normal process 
B)  is used in plant cell activation 
C)  must be used to produce animal bioreactors 
D)  occurs when creating a knockout animal 
E)  is a technique not proven to be successful 
A)  occurs during meiosis as a normal process 
B)  is used in plant cell activation 
C)  must be used to produce animal bioreactors 
D)  occurs when creating a knockout animal 
E)  is a technique not proven to be successful

Question 6

In what phase of clinical trials are animals used?

Accepted Answer

A)  Preclinical testing 
B)  Phase I 
C)  Phase II 
D)  Phase III 
E)  Post launch 
A)  Preclinical testing 
B)  Phase I 
C)  Phase II 
D)  Phase III 
E)  Post launch

Question 7

The transfer of DNA into eukaryotic cells is known as ________.

Accepted Answer

A)  transformation 
B)  transduction 
C)  transfection 
D)  transcription 
E)  translation 
A)  transformation 
B)  transduction 
C)  transfection 
D)  transcription 
E)  translation

Question 8

Which of the following transgenic techniques requires linker proteins?

Accepted Answer

A)  Embryonic stem cell method 
B)  Retrovirus-mediated method 
C)  Pronuclear injection method 
D)  Sperm-mediated transfer method 
E)  Plasma cell membrane fusion method 
A)  Embryonic stem cell method 
B)  Retrovirus-mediated method 
C)  Pronuclear injection method 
D)  Sperm-mediated transfer method 
E)  Plasma cell membrane fusion method

Question 9

In a gene knock-in experiment, ________.

Accepted Answer

A)  the effect is exactly the same as a knockout 
B)  the animal gene of interest is replaced with the human counterpart gene 
C)  the gene is non-functional 
D)  the embryo cannot survive to birth 
E)  the gene product is produced to at least twice the normal levels 
A)  the effect is exactly the same as a knockout 
B)  the animal gene of interest is replaced with the human counterpart gene 
C)  the gene is non-functional 
D)  the embryo cannot survive to birth 
E)  the gene product is produced to at least twice the normal levels

Question 10

An enucleated egg cell ________.

Accepted Answer

A)  contains an extra copy of a gene 
B)  has two nuclei 
C)  lacks a nucleus 
D)  cannot be used for cloning 
E)  is a type of stem cell 
A)  contains an extra copy of a gene 
B)  has two nuclei 
C)  lacks a nucleus 
D)  cannot be used for cloning 
E)  is a type of stem cell

Question 11

Explain the idea of transgenic animals as "bioreactors."

Accepted Answer

The biotechnology product is made in the

Question 12

Why did Dolly the sheep die at a young age?

Accepted Answer

Although Dolly was created by animal clo

Question 13

Monoclonal antibodies ________.

Accepted Answer

A)  are fairly non-specific 
B)  have not yet been approved by the FDA 
C)  have not been found to treat any disorders 
D)  are produced from myeloma cells only 
E)  are highly specific to a particular antigen 
A)  are fairly non-specific 
B)  have not yet been approved by the FDA 
C)  have not been found to treat any disorders 
D)  are produced from myeloma cells only 
E)  are highly specific to a particular antigen

Question 14

Which of the following approaches is not used to create a transgenic animal?

Accepted Answer

A)  Sperm-mediated DNA transfer 
B)  Pronuclear microinjection 
C)  Transfection of ES cells 
D)  Retrovirus-mediated transgenesis 
E)  Embryo twinning 
A)  Sperm-mediated DNA transfer 
B)  Pronuclear microinjection 
C)  Transfection of ES cells 
D)  Retrovirus-mediated transgenesis 
E)  Embryo twinning

Question 15

Which of the following transgenic techniques requires no vector to be used?

Accepted Answer

A)  Embryonic stem cell method 
B)  Retrovirus-mediated method 
C)  Pronuclear injection method 
D)  Sperm-mediated transfer method 
E)  Plasma cell membrane fusion method 
A)  Embryonic stem cell method 
B)  Retrovirus-mediated method 
C)  Pronuclear injection method 
D)  Sperm-mediated transfer method 
E)  Plasma cell membrane fusion method

Question 16

What is the purpose of using animals in clinical trials?

Accepted Answer

A)  To test the safety of a compound 
B)  To assess the biological activity of a compound 
C)  To decide how to produce (i.e., manufacture) the compound 
D)  A and B 
E)  A, B, and C 
A)  To test the safety of a compound 
B)  To assess the biological activity of a compound 
C)  To decide how to produce (i.e., manufacture) the compound 
D)  A and B 
E)  A, B, and C

Question 17

Carbon Copy is a ________.

Accepted Answer

A)  transgenic goat 
B)  cloned cow 
C)  chimera 
D)  cloned cat 
E)  None of these choices 
A)  transgenic goat 
B)  cloned cow 
C)  chimera 
D)  cloned cat 
E)  None of these choices

Question 18

What types of cells fuse together to form hybridomas for making monoclonal antibodies?

Accepted Answer

A)  Bacterial cells and T cells 
B)  Myeloma cells and B cells 
C)  Liver cells and antibody-forming cells 
D)  Monoclonal antibodies and myeloma cells 
E)  None of these choices 
A)  Bacterial cells and T cells 
B)  Myeloma cells and B cells 
C)  Liver cells and antibody-forming cells 
D)  Monoclonal antibodies and myeloma cells 
E)  None of these choices

Question 19

Embryo twinning refers to ________.

Accepted Answer

A)  a cloning step that involves enucleation 
B)  a cloning step that involves splitting embryos in half 
C)  a technique used in only primate cloning 
D)  a novel technique not yet employed by scientists 
E)  a cloning step that involves one half of an embryo 
A)  a cloning step that involves enucleation 
B)  a cloning step that involves splitting embryos in half 
C)  a technique used in only primate cloning 
D)  a novel technique not yet employed by scientists 
E)  a cloning step that involves one half of an embryo

Question 20

Which of the following techniques has been used to clone sheep, cats, cows, and several other types of mammals?

Accepted Answer

A)  DNA cloning 
B)  Somatic cell nuclear transfer 
C)  Therapeutic cloning 
D)  Electroporation 
E)  Gene knockout 
A)  DNA cloning 
B)  Somatic cell nuclear transfer 
C)  Therapeutic cloning 
D)  Electroporation 
E)  Gene knockout

One of the most common organisms used to produce knockouts is ________.

________ animals contain an inserted and functional gene from another source.

________ animals have been genetically engineered so that a specific gene is disrupted and rendered nonfunctional.

What are the three "Rs" related to regulation of animal research?

Homologous recombination ________.

In what phase of clinical trials are animals used?

The transfer of DNA into eukaryotic cells is known as ________.

Which of the following transgenic techniques requires linker proteins?

In a gene knock-in experiment, ________.

An enucleated egg cell ________.

Explain the idea of transgenic animals as "bioreactors."

Why did Dolly the sheep die at a young age?

Monoclonal antibodies ________.

Which of the following approaches is not used to create a transgenic animal?

Which of the following transgenic techniques requires no vector to be used?

What is the purpose of using animals in clinical trials?

Carbon Copy is a ________.

What types of cells fuse together to form hybridomas for making monoclonal antibodies?

Embryo twinning refers to ________.

Which of the following techniques has been used to clone sheep, cats, cows, and several other types of mammals?

The Biotechnology Century and Its Workforce

An Introduction to Genes and Genomes

Recombinant Dna Technology and Genomics

Proteins As Products

Microbial Biotechnology

Plant Biotechnology

Dna Fingerprinting and Forensic Analysis

Bioremediation

Aquatic Biotechnology

Medical Biotechnology

Biotechnology Regulations

Ethics and Biotechnology

Filters

Exam 7: Animal Biotechnology

One of the most common organisms used to produce knockouts is ________.

________ animals contain an inserted and functional gene from another source.

________ animals have been genetically engineered so that a specific gene is disrupted and rendered nonfunctional.

What are the three "Rs" related to regulation of animal research?

Homologous recombination ________.

In what phase of clinical trials are animals used?

The transfer of DNA into eukaryotic cells is known as ________.

Which of the following transgenic techniques requires linker proteins?

In a gene knock-in experiment, ________.

An enucleated egg cell ________.

Explain the idea of transgenic animals as "bioreactors."

Why did Dolly the sheep die at a young age?

Monoclonal antibodies ________.

Which of the following approaches is not used to create a transgenic animal?

Which of the following transgenic techniques requires no vector to be used?

What is the purpose of using animals in clinical trials?

Carbon Copy is a ________.

What types of cells fuse together to form hybridomas for making monoclonal antibodies?

Embryo twinning refers to ________.

Which of the following techniques has been used to clone sheep, cats, cows, and several other types of mammals?

The Biotechnology Century and Its Workforce

An Introduction to Genes and Genomes

Recombinant Dna Technology and Genomics

Proteins As Products

Microbial Biotechnology

Plant Biotechnology

Dna Fingerprinting and Forensic Analysis

Bioremediation

Aquatic Biotechnology

Medical Biotechnology

Biotechnology Regulations

Ethics and Biotechnology

Filters