Question 1

During a polymerase chain reaction,

Accepted Answer

A)  a DNA template is mixed with primers, nucleotides, and Taq polymerase. 
B)  the sample is heated in order to allow the two strands of DNA to separate completely. 
C)  some of the primers base-pair with template DNA. 
D)  Taq polymerase performs DNA synthesis from the primers. 
E)  all of these occur. 
A)  a DNA template is mixed with primers, nucleotides, and Taq polymerase. 
B)  the sample is heated in order to allow the two strands of DNA to separate completely. 
C)  some of the primers base-pair with template DNA. 
D)  Taq polymerase performs DNA synthesis from the primers. 
E)  all of these occur.

Question 2

Transgenic goats are now able to produce proteins used to treat

Accepted Answer

A)  cystic fibrosis. 
B)  heart attacks. 
C)  blood clotting disorders. 
D)  nerve gas exposure. 
E)  all of these. 
A)  cystic fibrosis. 
B)  heart attacks. 
C)  blood clotting disorders. 
D)  nerve gas exposure. 
E)  all of these.

Question 3

Which of the following is NOT true about restriction enzymes?

Accepted Answer

A)  Restriction enzymes recognize specific DNA sequences. 
B)  Restriction enzymes cut DNA independently of its origin. 
C)  Restriction enzymes recognize specific nucleotide sequences. 
D)  Restriction enzymes cut RNA or DNA sequences at specific sites. 
E)  More than one of these is NOT true. 
A)  Restriction enzymes recognize specific DNA sequences. 
B)  Restriction enzymes cut DNA independently of its origin. 
C)  Restriction enzymes recognize specific nucleotide sequences. 
D)  Restriction enzymes cut RNA or DNA sequences at specific sites. 
E)  More than one of these is NOT true.

Question 4

Which of the following molecules are able to cut DNA molecules at specific sequences?

Accepted Answer

A)  assembly enzymes 
B)  hydrolytic enzymes 
C)  restriction enzymes 
D)  ligase enzymes 
E)  repair enzymes 
A)  assembly enzymes 
B)  hydrolytic enzymes 
C)  restriction enzymes 
D)  ligase enzymes 
E)  repair enzymes

Question 5

Which of the following is NOT used or related to the creation of recombinant DNA?

Accepted Answer

A)  ligases 
B)  restiction enzymes 
C)  plasmids 
D)  SNPs 
E)  sticky ends 
A)  ligases 
B)  restiction enzymes 
C)  plasmids 
D)  SNPs 
E)  sticky ends

Question 6

Which of the following genetic problems is NOT being addressed by gene therapy?

Accepted Answer

A)  Down syndrome 
B)  cystic fibrosis 
C)  hemophilia 
D)  immune disorder 
E)  cancers 
A)  Down syndrome 
B)  cystic fibrosis 
C)  hemophilia 
D)  immune disorder 
E)  cancers

Question 7

Which of the following diseases has gene therapy been successful in curing?

Accepted Answer

A)  AIDS 
B)  SCIDS 
C)  diabetes 
D)  hemophilia A 
E)  more than one of these 
A)  AIDS 
B)  SCIDS 
C)  diabetes 
D)  hemophilia A 
E)  more than one of these

Question 8

Which of the following is a concern over genetically modified organisms?

Accepted Answer

A)  viral infections 
B)  tissue rejections 
C)  animal suffering 
D)  gene escape 
E)  all of these are concerns over genetically modified organisms 
A)  viral infections 
B)  tissue rejections 
C)  animal suffering 
D)  gene escape 
E)  all of these are concerns over genetically modified organisms

Question 9

After 30 cycles of PCR cloning of a segment of DNA,how many copies of that segment will there be?

Accepted Answer

A)  1000 
B)  10,000 
C)  100,000 
D)  1,000,000 
E)  1,000,000,000 
A)  1000 
B)  10,000 
C)  100,000 
D)  1,000,000 
E)  1,000,000,000

Question 10

Which of the following is TRUE of probes?

Accepted Answer

A)  they are artificially made 
B)  they are isolated from bacteria 
C)  they are the result of nucleic acid hybridization 
D)  they are isolated from SNPs 
E)  more than one of these is true 
A)  they are artificially made 
B)  they are isolated from bacteria 
C)  they are the result of nucleic acid hybridization 
D)  they are isolated from SNPs 
E)  more than one of these is true

Question 11

In gene therapy,what is transferred into an individual's body cells?

Accepted Answer

A)  new genomes 
B)  recombinant DNA 
C)  GMOs 
D)  bacterial plasmids 
E)  DNA libraries 
A)  new genomes 
B)  recombinant DNA 
C)  GMOs 
D)  bacterial plasmids 
E)  DNA libraries

Question 12

Four of the five answers listed below are involved in engineering and expressing a recombinant DNA molecule.Select the EXCEPTION.

Accepted Answer

A)  DNA ligase 
B)  DNA polymerase 
C)  restriction enzymes 
D)  plasmid 
E)  bacteria 
A)  DNA ligase 
B)  DNA polymerase 
C)  restriction enzymes 
D)  plasmid 
E)  bacteria

Question 13

The discovery of which of the following allowed researchers to determine the sequences of nucleotides in DNA molecules?

Accepted Answer

A)  single-nucleotide polymorphisms 
B)  restriction enzymes 
C)  DNA assembly enzymes 
D)  fragment length polymorphisms 
E)  receptor proteins 
A)  single-nucleotide polymorphisms 
B)  restriction enzymes 
C)  DNA assembly enzymes 
D)  fragment length polymorphisms 
E)  receptor proteins

Question 14

Gene therapy consist of

Accepted Answer

A)  replacing the genomes of an individual with a brand new set of genomes with the goal of treating a genetic defect. 
B)  replacing a defective gene in an individual with a normal gene with the goal of treating a genetic disorder. 
C)  transferring a modified gene into an individual with the goal of treating a genetic defect. 
D)  transferring a normal or a modified gene into an individual with the goal of treating a genetic defect. 
E)  replacing a defective enzyme in an individual with a normal enzyme with the goal of treating a genetic disorder. 
A)  replacing the genomes of an individual with a brand new set of genomes with the goal of treating a genetic defect. 
B)  replacing a defective gene in an individual with a normal gene with the goal of treating a genetic disorder. 
C)  transferring a modified gene into an individual with the goal of treating a genetic defect. 
D)  transferring a normal or a modified gene into an individual with the goal of treating a genetic defect. 
E)  replacing a defective enzyme in an individual with a normal enzyme with the goal of treating a genetic disorder.

Question 15

Classification.The following items are related to DNA recombinant technology.Answer the questions in reference to that topic.
a.restriction enzymes
b.recombinant DNA
c.plasmids
d.clones
e.DNA library
f.DNA polymerase
-collections of DNA fragments produced by restriction enzymes and incorporated into cloning vectors

Accepted Answer

The answer of Classification.The following items are related to DNA...

Question 16

Golden rice is made more beta-carotene rich with genes from bacteria and

Accepted Answer

A)  corn. 
B)  mice. 
C)  tulips. 
D)  daffodils. 
E)  oranges. 
A)  corn. 
B)  mice. 
C)  tulips. 
D)  daffodils. 
E)  oranges.

Question 17

In agriculture,the use of GMOs is regulated by

Accepted Answer

A)  the FDA 
B)  the AHA 
C)  Greenpeace 
D)  the USDA 
E)  the NIH 
A)  the FDA 
B)  the AHA 
C)  Greenpeace 
D)  the USDA 
E)  the NIH

Question 18

Classification.The following items are related to DNA recombinant technology.Answer the questions in reference to that topic.
a.restriction enzymes
b.recombinant DNA
c.plasmids
d.clones
e.DNA library
f.DNA polymerase
-DNA fragment that contains the DNA of more than one species

Accepted Answer

The answer of Classification.The following items are related to DNA...

Question 19

Beta- carotene is converted to vitamin ___ in the cells of the small intestine.

Accepted Answer

A)  B12 
B)  A 
C)  E 
D)  C 
E)  D 
A)  B12 
B)  A 
C)  E 
D)  C 
E)  D

Question 20

A plasmid is a

Accepted Answer

A)  small segment of DNA that is often isolated from virus chromosomes for cloning purposes. 
B)  large linear segment of DNA that is often isolated from bacterial chromosomes for cloning purposes. 
C)  small circular segment of DNA in bacteria that is linked by hydrogen bonds to the main bacterial chromosome. 
D)  large circular segment of DNA in bacteria that is completely separated with the chromosome. 
E)  small circular segment of DNA in bacteria that is completely separated from the chromosome. 
A)  small segment of DNA that is often isolated from virus chromosomes for cloning purposes. 
B)  large linear segment of DNA that is often isolated from bacterial chromosomes for cloning purposes. 
C)  small circular segment of DNA in bacteria that is linked by hydrogen bonds to the main bacterial chromosome. 
D)  large circular segment of DNA in bacteria that is completely separated with the chromosome. 
E)  small circular segment of DNA in bacteria that is completely separated from the chromosome.

Exam 10: Biotechnology

During a polymerase chain reaction,

Transgenic goats are now able to produce proteins used to treat

Which of the following is NOT true about restriction enzymes?

Which of the following molecules are able to cut DNA molecules at specific sequences?

Which of the following is NOT used or related to the creation of recombinant DNA?

Which of the following genetic problems is NOT being addressed by gene therapy?

Which of the following diseases has gene therapy been successful in curing?

Which of the following is a concern over genetically modified organisms?

After 30 cycles of PCR cloning of a segment of DNA,how many copies of that segment will there be?

Which of the following is TRUE of probes?

In gene therapy,what is transferred into an individual's body cells?

Four of the five answers listed below are involved in engineering and expressing a recombinant DNA molecule.Select the EXCEPTION.

The discovery of which of the following allowed researchers to determine the sequences of nucleotides in DNA molecules?

Gene therapy consist of

Golden rice is made more beta-carotene rich with genes from bacteria and

In agriculture,the use of GMOs is regulated by

Classification.The following items are related to DNA recombinant technology.Answer the questions in reference to that topic. a.restriction enzymes b.recombinant DNA c.plasmids d.clones e.DNA library f.DNA polymerase -DNA fragment that contains the DNA of more than one species

Beta- carotene is converted to vitamin ___ in the cells of the small intestine.

A plasmid is a

Invitation to Biology

Molecules of Life

Cell Structure

Energy and Metabolism

Capturing and Releasing Energy

DNA Structure and Function

Gene Expression and Control

How Cells Reproduce

Patterns of Inheritance

Evidence of Evolution

Processes of Evolution

Early Life Forms and the Viruses

Plants and Fungi

Animal Evolution

Population Ecology

Communities and Ecosystems

The Biosphere and Human Effects

Animal Tissues and Organs

How Animals Move

Circulation and Respiration

Immunity

Digestion and Excretion

Neural Control and the Senses

Endocrine Control

Reproduction and Development

Plant Form and Function

Plant Reproduction and Development

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