Question 1

The E.coli chromosome is

Accepted Answer

A) linear. 
B) circular. 
C) single-stranded. 
D) less than a megabase in length. 
E) compartmentalized within an intracellular membrane. 
A) linear. 
B) circular. 
C) single-stranded. 
D) less than a megabase in length. 
E) compartmentalized within an intracellular membrane.

Question 2

During a cis-trans complementation assay,two lac- strains of bacteria are crossed.The progeny cells exhibit a lac- phenotype.What does this indicate?

Accepted Answer

The two mutations are in diffe

Question 3

The reason that many plasmids used in laboratories for transformation experiments contain an ampicillin resistance gene (ampR)is

Accepted Answer

A) so that the plasmid can replicate in bacterial cells. 
B) so that the plasmid can recombine with the bacterial genome. 
C) to enable the recipient cell to conjugate. 
D) to provide a selectable marker. 
E) to map point mutations. 
A) so that the plasmid can replicate in bacterial cells. 
B) so that the plasmid can recombine with the bacterial genome. 
C) to enable the recipient cell to conjugate. 
D) to provide a selectable marker. 
E) to map point mutations.

Question 4

An arg- strain of E.coli is transformed with a plasmid carrying the wild-type (arg+)gene.The transformed cells are replica-plated to two minimal medium plates: one supplemented with arginine and one lacking arginine.The transformed cells would grow on

Accepted Answer

A) only the plate supplemented with arginine. 
B) only the plate lacking arginine. 
C) both plates. 
D) neither plate. 
E) the plate supplemented with arginine,but only if a mutation occurred. 
A) only the plate supplemented with arginine. 
B) only the plate lacking arginine. 
C) both plates. 
D) neither plate. 
E) the plate supplemented with arginine,but only if a mutation occurred.

Question 5

What are temperate bacteriophages?

Accepted Answer

Phages that can alte

Question 6

Which of the following methods can be used to create competent bacterial cells?

Accepted Answer

A) Treating the cells chemically 
B) Exposing cells to a strong electric field 
C) Making the cell membrane more permeable to DNA 
D) Allowing the culture to enter stationary phase growth 
E) A,B,and C only 
A) Treating the cells chemically 
B) Exposing cells to a strong electric field 
C) Making the cell membrane more permeable to DNA 
D) Allowing the culture to enter stationary phase growth 
E) A,B,and C only

Question 7

Cotransductant bacteria can occur if

Accepted Answer

A) a bacterial cell picks up two fragments of DNA from the environment,each with a different gene. 
B) double crossover occurs between prophage DNA and the bacterial chromosome. 
C) two genes are closely linked enough so that they can be packaged into a phage head and injected into a cell by a single phage. 
D) two genes are introduced into the same bacterium by simultaneous infection with two different phages. 
E) Both C and D 
A) a bacterial cell picks up two fragments of DNA from the environment,each with a different gene. 
B) double crossover occurs between prophage DNA and the bacterial chromosome. 
C) two genes are closely linked enough so that they can be packaged into a phage head and injected into a cell by a single phage. 
D) two genes are introduced into the same bacterium by simultaneous infection with two different phages. 
E) Both C and D

Question 8

Cotransductants can be detected when the transduced genes are

Accepted Answer

A) closely linked. 
B) far apart on the same chromosome. 
C) on different chromosomes. 
D) mutant. 
E) Both B and D 
A) closely linked. 
B) far apart on the same chromosome. 
C) on different chromosomes. 
D) mutant. 
E) Both B and D

Question 9

In the case of genetic exchange in bacteria by transformation,there is an exchange of DNA back and forth between cells,and a complete diploid cell is formed.

Accepted Answer

A) True 
 B)False

Question 10

"Hot spots" are locations in a gene where mutations are found at a relatively high frequency.

Accepted Answer

A) True 
 B)False

Question 11

Which of the following has episomal DNA inserted in the cell's chromosome?

Accepted Answer

A) An F+ cell 
B) An F' plasmid 
C) An Hfr cell 
D) An F- cell 
E) All except D 
A) An F+ cell 
B) An F' plasmid 
C) An Hfr cell 
D) An F- cell 
E) All except D

Question 12

A merodiploid cell has two copies of one or a few genes and only one copy of all the others.

Accepted Answer

A) True 
 B)False

Question 13

E.coli strains that are Hfr

Accepted Answer

A) are easily mutated. 
B) are susceptible to infection by bacteriophage. 
C) contain the F factor integrated in the bacterial chromosome. 
D) cannot be made competent. 
E) have a low frequency of recombination. 
A) are easily mutated. 
B) are susceptible to infection by bacteriophage. 
C) contain the F factor integrated in the bacterial chromosome. 
D) cannot be made competent. 
E) have a low frequency of recombination.

Question 14

How did Seymour Benzer use deletion mapping to construct fine-scale maps of the rII region of the T4 bacteriophage genome?

Accepted Answer

He used deletion mutants,which,unlike po

Question 15

________ bacterial strains require nutritional supplements to grow in a minimal medium.

Accepted Answer

A) Autotrophic 
B) Prototrophic 
C) Phototrophic 
D) Heterotrophic 
E) Auxotrophic 
A) Autotrophic 
B) Prototrophic 
C) Phototrophic 
D) Heterotrophic 
E) Auxotrophic

Question 16

In F+ × F- crosses,none of the bacterial chromosome is transferred; only the F factor is.

Accepted Answer

A) True 
 B)False

Question 17

Which of the following statements is true regarding a temperate phage?

Accepted Answer

A) It can reproduce at warm or cool temperatures. 
B) It is required for transformation. 
C) It can be lytic or lysogenic. 
D) It causes bacterial cells to conjugate. 
E) It reproduces by binary fission. 
A) It can reproduce at warm or cool temperatures. 
B) It is required for transformation. 
C) It can be lytic or lysogenic. 
D) It causes bacterial cells to conjugate. 
E) It reproduces by binary fission.

Question 18

A plasmid such as an F factor that is capable of integrating into the bacterial chromosome is a(n)

Accepted Answer

A) prophage. 
B) episome. 
C) auxisome. 
D) perisome. 
E) elaiosome. 
A) prophage. 
B) episome. 
C) auxisome. 
D) perisome. 
E) elaiosome.

Question 19

In E.coli,λ DNA integrates into which site on the bacterial chromosome?

Accepted Answer

A) ori 
B) trp 
C) bio 
D) gal 
E) att 
A) ori 
B) trp 
C) bio 
D) gal 
E) att

Question 20

Conjugation by which of the following with an F- cell results in a cell that remains F-?

Accepted Answer

A) F+  
B) Hfr 
C) F 
D) A and C 
E) B and C 
A) F+  
B) Hfr 
C) F 
D) A and C 
E) B and C

Exam 15: Genetics of Bacteria and Bacteriophages

The E.coli chromosome is

During a cis-trans complementation assay,two lac- strains of bacteria are crossed.The progeny cells exhibit a lac- phenotype.What does this indicate?

The reason that many plasmids used in laboratories for transformation experiments contain an ampicillin resistance gene (ampR)is

An arg- strain of E.coli is transformed with a plasmid carrying the wild-type (arg+)gene.The transformed cells are replica-plated to two minimal medium plates: one supplemented with arginine and one lacking arginine.The transformed cells would grow on

What are temperate bacteriophages?

Which of the following methods can be used to create competent bacterial cells?

Cotransductant bacteria can occur if

Cotransductants can be detected when the transduced genes are

In the case of genetic exchange in bacteria by transformation,there is an exchange of DNA back and forth between cells,and a complete diploid cell is formed.

"Hot spots" are locations in a gene where mutations are found at a relatively high frequency.

Which of the following has episomal DNA inserted in the cell's chromosome?

A merodiploid cell has two copies of one or a few genes and only one copy of all the others.

E.coli strains that are Hfr

How did Seymour Benzer use deletion mapping to construct fine-scale maps of the rII region of the T4 bacteriophage genome?

________ bacterial strains require nutritional supplements to grow in a minimal medium.

In F+ × F- crosses,none of the bacterial chromosome is transferred; only the F factor is.

Which of the following statements is true regarding a temperate phage?

A plasmid such as an F factor that is capable of integrating into the bacterial chromosome is a(n)

In E.coli,λ DNA integrates into which site on the bacterial chromosome?

Conjugation by which of the following with an F- cell results in a cell that remains F-?

Genetics: An Introduction

DNA: The Genetic Material

DNA Replication

Gene Function

Gene Expression: Transcription

Gene Expression: Translation

Dna Mutation, DNA Repair, and Transposable Elements

Genomics: The Mapping and Sequencing of Genomes

Functional and Comparative Genomics

Recombinant DNA Technology

Mendelian Genetics

Chromosomal Basis of Inheritance

Extensions of and Deviations From Mendelian Genetic Principles

Genetic Mapping in Eukaryotes

Variations in Chromosome Structure and Number

Regulation of Gene Expression in Bacteria and Bacteriophages

Regulation of Gene Expression in Eukaryotes

Genetic Analysis of Development

Genetics of Cancer

Population Genetics

Quantitative Genetics

Molecular Evolution

Filters

During a cis-trans complementation assay,two lac^- strains of bacteria are crossed.The progeny cells exhibit a lac^- phenotype.What does this indicate?

The reason that many plasmids used in laboratories for transformation experiments contain an ampicillin resistance gene (amp_R)is

An arg^- strain of E.coli is transformed with a plasmid carrying the wild-type (arg⁺)gene.The transformed cells are replica-plated to two minimal medium plates: one supplemented with arginine and one lacking arginine.The transformed cells would grow on

In F⁺ × F^- crosses,none of the bacterial chromosome is transferred; only the F factor is.

Conjugation by which of the following with an F^- cell results in a cell that remains F^-?