Question 1

What are some key differences in replication between E.coli DNA and λ phage DNA?

Accepted Answer

Both start replication as a circular mol

Question 2

How will DNA replication be affected if DNA polymerase I has a mutation that inactivates 5'-to-3' exonuclease activity?

Accepted Answer

The 5'-to-3' exonuclease activity of DNA

Question 3

What are the key replication enzymes at the replisome,and how is DNA replication on both leading and lagging strands made efficient through the conformation of the DNA at the replisome in prokaryotes?

Accepted Answer

The key replication enzymes at the repli

Question 4

Which kind of enzyme prevents DNA from tangling up by introducing negative supercoils as the replication fork migrates during replication?

Accepted Answer

A) Helicase 
B) Ligase 
C) DNA polymerase I 
D) DNA polymerase III 
E) Topoisomerase 
A) Helicase 
B) Ligase 
C) DNA polymerase I 
D) DNA polymerase III 
E) Topoisomerase

Question 5

DNA replication is always

Accepted Answer

A) discontinuous. 
B) bidirectional. 
C) conservative. 
D) semiconservative. 
E) dispersive. 
A) discontinuous. 
B) bidirectional. 
C) conservative. 
D) semiconservative. 
E) dispersive.

Question 6

Which enzyme elongates the new DNA strand starting at an RNA primer?

Accepted Answer

A) DNA polymerase I 
B) DNA polymerase III 
C) RNA primase 
D) DNA ligase 
E) RNA polymerase 
A) DNA polymerase I 
B) DNA polymerase III 
C) RNA primase 
D) DNA ligase 
E) RNA polymerase

Question 7

During replication,the direction of synthesis of new DNA from the leading and lagging strands is

Accepted Answer

A) 5' to 3' only. 
B) 3' to 5' only. 
C) from left to right only. 
D) both 5' to 3' and 3' to 5'. 
E) different,depending on whether the cell is prokaryotic or eukaryotic. 
A) 5' to 3' only. 
B) 3' to 5' only. 
C) from left to right only. 
D) both 5' to 3' and 3' to 5'. 
E) different,depending on whether the cell is prokaryotic or eukaryotic.

Question 8

How many replication forks are produced when DNA denatures at an origin?

Accepted Answer

A) 0 
B) 1 
C) 2 
D) 3 
E) The number varies 
A) 0 
B) 1 
C) 2 
D) 3 
E) The number varies

Question 9

In E.coli,replication begins at which chromosome site?

Accepted Answer

A) The replication fork 
B) ter 
C) oriC 
D) TBP 
E) All of these 
A) The replication fork 
B) ter 
C) oriC 
D) TBP 
E) All of these

Question 10

Mg2+ ions are required for optimal DNA polymerase activity.

Accepted Answer

A) True 
 B)False

Question 11

The diploid set of chromosomes in Drosophila embryos replicates six times faster than the single E.coli chromosome,even though there is about 100 times more DNA in Drosophila than in E.coli and the rate of movement of the replication fork in Drosophila is much slower.How is this so?

Accepted Answer

Eukaryotic chromosomes duplicate rapidly

Question 12

Only the leading strand of a DNA molecule serves as a template during replication.

Accepted Answer

A) True 
 B)False

Question 13

Describe the method devised by Arthur Kornberg which first successfully achieved DNA synthesis in vitro,including its components and their uses.

Accepted Answer

Kornberg mixed together DNA fragments,al

Question 14

DNA polymerase III is very inaccurate at matching bases during replication,with errors in one out of every 100 base pairs.

Accepted Answer

A) True 
 B)False

Question 15

Rolling circle replication of DNA is characterized by the absence of

Accepted Answer

A) the DNA polymerase. 
B) a nick in the DNA template. 
C) the RNA primers. 
D) the replication bubble. 
E) the Okazaki fragments. 
A) the DNA polymerase. 
B) a nick in the DNA template. 
C) the RNA primers. 
D) the replication bubble. 
E) the Okazaki fragments.

Question 16

A cross is made between yeast cells with different alleles for a set of linked genes: pr+q × p+rq+.The resulting tetrads show a 3:1 ratio for r+ to r instead of the expected 2:2 ratio.Can you explain how this could have occurred?

Accepted Answer

Gene conversion by a mismatch repair mec

Question 17

After a region of DNA has been replicated,________ removes the RNA primers.

Accepted Answer

A) DNA polymerase I 
B) DNA polymerase III 
C) DNA helicase 
D) RNA primase 
E) DNA ligase 
A) DNA polymerase I 
B) DNA polymerase III 
C) DNA helicase 
D) RNA primase 
E) DNA ligase

Question 18

In eukaryotic cells,histone proteins are actively synthesized during the S phase of the cell cycle.

Accepted Answer

A) True 
 B)False

Question 19

Match between columns
                        Premises: Replicon
Primosome
Telomerase
Replicator
Replisome

Accepted Answer

The Answer of A complex of helicase and primase on is...

Question 20

As helicase unwinds the DNA molecule,what keeps the strands apart?

Accepted Answer

A) DNA polymerase 
B) Reverse transcriptase 
C) Replication fork 
D) Single-strand binding proteins 
E) Okazaki fragments 
A) DNA polymerase 
B) Reverse transcriptase 
C) Replication fork 
D) Single-strand binding proteins 
E) Okazaki fragments

Exam 3: DNA Replication

What are some key differences in replication between E.coli DNA and λ phage DNA?

How will DNA replication be affected if DNA polymerase I has a mutation that inactivates 5'-to-3' exonuclease activity?

What are the key replication enzymes at the replisome,and how is DNA replication on both leading and lagging strands made efficient through the conformation of the DNA at the replisome in prokaryotes?

Which kind of enzyme prevents DNA from tangling up by introducing negative supercoils as the replication fork migrates during replication?

DNA replication is always

Which enzyme elongates the new DNA strand starting at an RNA primer?

During replication,the direction of synthesis of new DNA from the leading and lagging strands is

How many replication forks are produced when DNA denatures at an origin?

In E.coli,replication begins at which chromosome site?

Mg2+ ions are required for optimal DNA polymerase activity.

The diploid set of chromosomes in Drosophila embryos replicates six times faster than the single E.coli chromosome,even though there is about 100 times more DNA in Drosophila than in E.coli and the rate of movement of the replication fork in Drosophila is much slower.How is this so?

Only the leading strand of a DNA molecule serves as a template during replication.

Describe the method devised by Arthur Kornberg which first successfully achieved DNA synthesis in vitro,including its components and their uses.

DNA polymerase III is very inaccurate at matching bases during replication,with errors in one out of every 100 base pairs.

Rolling circle replication of DNA is characterized by the absence of

A cross is made between yeast cells with different alleles for a set of linked genes: pr+q × p+rq+.The resulting tetrads show a 3:1 ratio for r+ to r instead of the expected 2:2 ratio.Can you explain how this could have occurred?

After a region of DNA has been replicated,________ removes the RNA primers.

In eukaryotic cells,histone proteins are actively synthesized during the S phase of the cell cycle.

As helicase unwinds the DNA molecule,what keeps the strands apart?

Genetics: An Introduction

DNA: The Genetic Material

Gene Function

Gene Expression: Transcription

Gene Expression: Translation

Dna Mutation, DNA Repair, and Transposable Elements

Genomics: The Mapping and Sequencing of Genomes

Functional and Comparative Genomics

Recombinant DNA Technology

Mendelian Genetics

Chromosomal Basis of Inheritance

Extensions of and Deviations From Mendelian Genetic Principles

Genetic Mapping in Eukaryotes

Genetics of Bacteria and Bacteriophages

Variations in Chromosome Structure and Number

Regulation of Gene Expression in Bacteria and Bacteriophages

Regulation of Gene Expression in Eukaryotes

Genetic Analysis of Development

Genetics of Cancer

Population Genetics

Quantitative Genetics

Molecular Evolution

Filters

Mg²⁺ ions are required for optimal DNA polymerase activity.

A cross is made between yeast cells with different alleles for a set of linked genes: pr^+q × p^+rq+.The resulting tetrads show a 3:1 ratio for r⁺to r instead of the expected 2:2 ratio.Can you explain how this could have occurred?