Question 1

Quality control tests for spectrophotometers consist of checking wavelength accuracy.

Accepted Answer

A) True 
 B)False  True

Question 2

An analytic method that depends on light-scattering properties of antigen-antibody complexes is:

Accepted Answer

A)  absorption spectrophotometry. 
B)  reflectance spectrophotometry. 
C)  nephelometry. 
D)  ion-selective electrodes. 
A)  absorption spectrophotometry. 
B)  reflectance spectrophotometry. 
C)  nephelometry. 
D)  ion-selective electrodes. C

Question 3

Quality control tests for spectrophotometers consist of checking nonlinearity.

Accepted Answer

A) True 
 B)False  False

Question 4

Various enzymes are used in enzyme immunoassay.In order to be used,an enzyme must fulfill certain criteria,including no alteration by inhibitor with the system.

Accepted Answer

A) True 
 B)False

Question 5

The principle of flow cytometry is based on the fact that:

Accepted Answer

A)  cells have an electrical charge. 
B)  proteins can be precipitated. 
C)  cells are stained in suspension with an appropriate fluorochrome. 
D)  fluorescent dyes are nonspecific. 
A)  cells have an electrical charge. 
B)  proteins can be precipitated. 
C)  cells are stained in suspension with an appropriate fluorochrome. 
D)  fluorescent dyes are nonspecific.

Question 6

One criterion for a good standard curve is that the line must connect two points.

Accepted Answer

A) True 
 B)False

Question 7

The analytic technology that relies on direct measurement of electrical potential caused by the activity of free ions is:

Accepted Answer

A)  pH meter. 
B)  ion-selective electrodes (ISEs). 
C)  chromatography. 
D)  gel electrophoresis. 
A)  pH meter. 
B)  ion-selective electrodes (ISEs). 
C)  chromatography. 
D)  gel electrophoresis.

Question 8

In chromatography:

Accepted Answer

A)  mixtures of solutes dissolved in a common solvent are separated from one another. 
B)  mixtures of solvents are separated from one another. 
C)  solutions with various pH measurements are separated from one another. 
D)  either b or c are true. 
A)  mixtures of solutes dissolved in a common solvent are separated from one another. 
B)  mixtures of solvents are separated from one another. 
C)  solutions with various pH measurements are separated from one another. 
D)  either b or c are true.

Question 9

The piece of equipment that measures the H+ is:

Accepted Answer

A)  pH meter. 
B)  photometer. 
C)  spectrophotometer. 
D)  flow cytometer. 
A)  pH meter. 
B)  photometer. 
C)  spectrophotometer. 
D)  flow cytometer.

Question 10

Immunoassays depend on:

Accepted Answer

A)  antigen solubility. 
B)  antibody complexity. 
C)  antigen-antibody reactions. 
D)  antibody solubility. 
A)  antigen solubility. 
B)  antibody complexity. 
C)  antigen-antibody reactions. 
D)  antibody solubility.

Question 11

Match the approximate wavelength with the observed color. Use an answer only once.
-440-500

Accepted Answer

A) not visible (infrared light) 
B) red 
C) orange 
D) yellow 
E) green
F)blue
G)violet
H)not visible (ultraviolet light) 
A) not visible (infrared light) 
B) red 
C) orange 
D) yellow 
E) green
F)blue
G)violet
H)not visible (ultraviolet light)

Question 12

Beer's law states:

Accepted Answer

A)  the concentration of a substance is directly proportional to the amount of light absorbed. 
B)  inversely proportional to the logarithm of the transmitted light. 
C)  inversely proportional to the amount of light absorbed. 
D)  both a and b. 
A)  the concentration of a substance is directly proportional to the amount of light absorbed. 
B)  inversely proportional to the logarithm of the transmitted light. 
C)  inversely proportional to the amount of light absorbed. 
D)  both a and b.

Question 13

One of the most frequently used techniques in the clinical laboratory is:

Accepted Answer

A)  spectrophotometry. 
B)  chemiluminescence. 
C)  nephelometry. 
D)  electrophoresis. 
A)  spectrophotometry. 
B)  chemiluminescence. 
C)  nephelometry. 
D)  electrophoresis.

Question 14

One criterion for a good standard curve is that the line is straight.

Accepted Answer

A) True 
 B)False

Question 15

A popular at-home application of reflectance spectrophotometry in point-of-care testing (POCT)is:

Accepted Answer

A)  blood volume estimation. 
B)  drug monitoring. 
C)  glucose monitoring. 
D)  pH determination. 
A)  blood volume estimation. 
B)  drug monitoring. 
C)  glucose monitoring. 
D)  pH determination.

Question 16

Match the approximate wavelength with the observed color. Use an answer only once.
->750

Accepted Answer

A) not visible (infrared light) 
B) red 
C) orange 
D) yellow 
E) green
F)blue
G)violet
H)not visible (ultraviolet light) 
A) not visible (infrared light) 
B) red 
C) orange 
D) yellow 
E) green
F)blue
G)violet
H)not visible (ultraviolet light)

Question 17

A newer analytic technology that is becoming the technology of choice by most immunodiagnostic manufacturers is:

Accepted Answer

A)  chemiluminescence. 
B)  electrophoresis. 
C)  spectrophotometry. 
D)  enzyme immunoassay. 
A)  chemiluminescence. 
B)  electrophoresis. 
C)  spectrophotometry. 
D)  enzyme immunoassay.

Question 18

Spectrophotometry measures the concentration of an unknown substance by:

Accepted Answer

A)  counting the number of particles in a solution. 
B)  comparing an unknown colored substance to a standard solution. 
C)  measuring the electrical voltage generated in a chemical reaction. 
D)  measuring the amount of heat generated in a chemical reaction. 
A)  counting the number of particles in a solution. 
B)  comparing an unknown colored substance to a standard solution. 
C)  measuring the electrical voltage generated in a chemical reaction. 
D)  measuring the amount of heat generated in a chemical reaction.

Question 19

Fundamental prerequisite for a nephelometry measurement is:

Accepted Answer

A)  formation of a macromolecular complex. 
B)  the reaction between the protein being assayed and a specific antisera. 
C)  formation of an insoluble complex. 
D)  all of the above. 
A)  formation of a macromolecular complex. 
B)  the reaction between the protein being assayed and a specific antisera. 
C)  formation of an insoluble complex. 
D)  all of the above.

Question 20

Match the approximate wavelength with the observed color. Use an answer only once.
-620-750

Accepted Answer

A) not visible (infrared light) 
B) red 
C) orange 
D) yellow 
E) green
F)blue
G)violet
H)not visible (ultraviolet light) 
A) not visible (infrared light) 
B) red 
C) orange 
D) yellow 
E) green
F)blue
G)violet
H)not visible (ultraviolet light)

Quality control tests for spectrophotometers consist of checking wavelength accuracy.

An analytic method that depends on light-scattering properties of antigen-antibody complexes is:

Quality control tests for spectrophotometers consist of checking nonlinearity.

Various enzymes are used in enzyme immunoassay.In order to be used,an enzyme must fulfill certain criteria,including no alteration by inhibitor with the system.

The principle of flow cytometry is based on the fact that:

One criterion for a good standard curve is that the line must connect two points.

The analytic technology that relies on direct measurement of electrical potential caused by the activity of free ions is:

In chromatography:

The piece of equipment that measures the H+ is:

Immunoassays depend on:

Match the approximate wavelength with the observed color. Use an answer only once. -440-500

Beer's law states:

One of the most frequently used techniques in the clinical laboratory is:

One criterion for a good standard curve is that the line is straight.

A popular at-home application of reflectance spectrophotometry in point-of-care testing (POCT)is:

Match the approximate wavelength with the observed color. Use an answer only once. ->750

A newer analytic technology that is becoming the technology of choice by most immunodiagnostic manufacturers is:

Spectrophotometry measures the concentration of an unknown substance by:

Fundamental prerequisite for a nephelometry measurement is:

Match the approximate wavelength with the observed color. Use an answer only once. -620-750

Fundamentals of the Clinical Laboratory

Safety in the Clinical Laboratory

Quality Assessment and Quality Control in the Clinical Laboratory

Phlebotomy: Collecting and Processing Blood

The Microscope

Systems of Measurement, laboratory Equipment, and Reagents

Laboratory Mathematics and Solution Preparation

Delivery of Laboratory Testing From Point of Care to Total Automation

Introduction to Clinical Chemistry

Principles and Practice of Clinical Hematology

Introduction to Hemostasis

Renal Physiology and Urinalysis

Examination of Body Fluids and Miscellaneous Specimens

Introduction to Microbiology

Immunology and Serology

Immunohematology and Transfusion Medicine

Filters

Exam 8: Basic and New Techniques in the Clinical Laboratory

Quality control tests for spectrophotometers consist of checking wavelength accuracy.

An analytic method that depends on light-scattering properties of antigen-antibody complexes is:

Quality control tests for spectrophotometers consist of checking nonlinearity.

Various enzymes are used in enzyme immunoassay.In order to be used,an enzyme must fulfill certain criteria,including no alteration by inhibitor with the system.

The principle of flow cytometry is based on the fact that:

One criterion for a good standard curve is that the line must connect two points.

The analytic technology that relies on direct measurement of electrical potential caused by the activity of free ions is:

In chromatography:

The piece of equipment that measures the H+ is:

Immunoassays depend on:

Match the approximate wavelength with the observed color. Use an answer only once. -440-500

Beer's law states:

One of the most frequently used techniques in the clinical laboratory is:

One criterion for a good standard curve is that the line is straight.

A popular at-home application of reflectance spectrophotometry in point-of-care testing (POCT)is:

Match the approximate wavelength with the observed color. Use an answer only once. ->750

A newer analytic technology that is becoming the technology of choice by most immunodiagnostic manufacturers is:

Spectrophotometry measures the concentration of an unknown substance by:

Fundamental prerequisite for a nephelometry measurement is:

Match the approximate wavelength with the observed color. Use an answer only once. -620-750

Fundamentals of the Clinical Laboratory

Safety in the Clinical Laboratory

Quality Assessment and Quality Control in the Clinical Laboratory

Phlebotomy: Collecting and Processing Blood

The Microscope

Systems of Measurement, laboratory Equipment, and Reagents

Laboratory Mathematics and Solution Preparation

Delivery of Laboratory Testing From Point of Care to Total Automation

Introduction to Clinical Chemistry

Principles and Practice of Clinical Hematology

Introduction to Hemostasis

Renal Physiology and Urinalysis

Examination of Body Fluids and Miscellaneous Specimens

Introduction to Microbiology

Immunology and Serology

Immunohematology and Transfusion Medicine

Filters