Question 1

Why are cDNA libraries desirable for the expression of eukaryotic genes in prokaryotes?

Accepted Answer

With a cDNA library, the gene

Question 2

Identifying an individual by his or her DNA is referred to as ____.

Accepted Answer

A)  ​structural genomics 
B)  ​genomic fingerprinting 
C)  ​RNAprofiling 
D)  ​DNA profiling 
E)  ​individual genomics 
A)  ​structural genomics 
B)  ​genomic fingerprinting 
C)  ​RNAprofiling 
D)  ​DNA profiling 
E)  ​individual genomics

Question 3

cDNA ____.

Accepted Answer

A)  ​does not contain exons 
B)  ​is produced from tRNA 
C)  ​production utilizes reverse transcriptase 
D)  ​begins as a hybrid between two mRNA molecules 
E)  ​is present only in bacteria 
A)  ​does not contain exons 
B)  ​is produced from tRNA 
C)  ​production utilizes reverse transcriptase 
D)  ​begins as a hybrid between two mRNA molecules 
E)  ​is present only in bacteria

Question 4

To answer the following questions, refer to the five items listed below:
-The combination of a foreign piece of DNA with the DNA of another organism.

Accepted Answer

A) restriction enzymes 
B) recombinant DNA 
C) plasmids 
D) clones 
E) restriction sites 
A) restriction enzymes 
B) recombinant DNA 
C) plasmids 
D) clones 
E) restriction sites

Question 5

To answer the following questions refer to the four items listed below.
-This is an enzyme used to produce cDNA.

Accepted Answer

A) cDNA 
B) ​restriction enzymes 
C) reverse transcriptase 
D) ​DNA library 
A) cDNA 
B) ​restriction enzymes 
C) reverse transcriptase 
D) ​DNA library

Question 6

Figure 15.2
​
   
The following questionsrefer to the figure above illustrating the initial steps in the formation of recombinant DNA.
-In panel 4, the base-paired DNA fragments are joined together with ____.

Accepted Answer

A)  ​DNA polymerase 
B)  ​RNA polymerase 
C)  ​reverse transcriptase 
D)  ​DNA ligase 
E)  ​restriction endonuclease 
A)  ​DNA polymerase 
B)  ​RNA polymerase 
C)  ​reverse transcriptase 
D)  ​DNA ligase 
E)  ​restriction endonuclease

Question 7

Figure 15.5
  
-In step 2 (above) in the identification of the colony that contains a targeted gene, ____.

Accepted Answer

A)  ​bacterial cells are cloned 
B)  ​bacterial colonies are transferred 
C)  ​bacterial cells are ruptured 
D)  ​DNA is lysed 
E)  ​cells with the targeted gene are identified 
A)  ​bacterial cells are cloned 
B)  ​bacterial colonies are transferred 
C)  ​bacterial cells are ruptured 
D)  ​DNA is lysed 
E)  ​cells with the targeted gene are identified

Question 8

Seed banks ____.

Accepted Answer

A)  ​provide a source of seeds for farmers 
B)  ​preserve seeds for museums 
C)  ​preserve plant genetic diversity 
D)  ​distribute seeds to member seed companies 
E)  ​help to create additional plant diversity 
A)  ​provide a source of seeds for farmers 
B)  ​preserve seeds for museums 
C)  ​preserve plant genetic diversity 
D)  ​distribute seeds to member seed companies 
E)  ​help to create additional plant diversity

Question 9

Restriction enzymes ____.

Accepted Answer

A)  ​work at recognition sites 
B)  ​function only at "sticky ends" 
C)  ​produce uniform lengths of DNA 
D)  ​function in viruses 
E)  ​are produced by viruses to break down bacterial DNA 
A)  ​work at recognition sites 
B)  ​function only at "sticky ends" 
C)  ​produce uniform lengths of DNA 
D)  ​function in viruses 
E)  ​are produced by viruses to break down bacterial DNA

Question 10

__________ can reveal the order of nucleotide bases in a fragment of DNA.

Accepted Answer

The answer of __________ can reveal the order of nucleotide...

Question 11

Genetic engineering has produced which of the following animals?​

Accepted Answer

A)  ​wild caught salmon 
B)  ​pigs 
C)  ​Dolly the sheep 
D)  ​cows resistant to mad cow disease 
E)  ​grass-fed beef 
A)  ​wild caught salmon 
B)  ​pigs 
C)  ​Dolly the sheep 
D)  ​cows resistant to mad cow disease 
E)  ​grass-fed beef

Question 12

Today's automated DNA sequencing machines can sequence an individual's entire genome in ____.

Accepted Answer

A)  ​2 to 4 hours 
B)  ​8 to 24 hours 
C)  ​2 to 3 days 
D)  ​7 to 10 days 
E)  ​2 to 3 weeks 
A)  ​2 to 4 hours 
B)  ​8 to 24 hours 
C)  ​2 to 3 days 
D)  ​7 to 10 days 
E)  ​2 to 3 weeks

Question 13

Small circular molecules of "extra" DNA in bacteria ____.

Accepted Answer

A)  ​are plasmids 
B)  ​are DNA fragments from their main chromosome 
C)  ​result from the activity of restriction enzymes 
D)  ​are eventually degraded 
E)  ​cannot survive outside of the cell 
A)  ​are plasmids 
B)  ​are DNA fragments from their main chromosome 
C)  ​result from the activity of restriction enzymes 
D)  ​are eventually degraded 
E)  ​cannot survive outside of the cell

Question 14

________, a viral enzyme, is used to convert single-stranded RNA into ___________ for cloning.

Accepted Answer

Reverse tr

Question 15

For the polymerase chain reaction to occur, ____.

Accepted Answer

A)  ​isolated DNA molecules must be primed 
B)  ​all DNA fragments must be identical 
C)  ​the DNA must remain double stranded 
D)  ​a sticky end must be available for the ligase enzyme to function 
E)  ​a large sample of DNA is needed 
A)  ​isolated DNA molecules must be primed 
B)  ​all DNA fragments must be identical 
C)  ​the DNA must remain double stranded 
D)  ​a sticky end must be available for the ligase enzyme to function 
E)  ​a large sample of DNA is needed

Question 16

In order for DNA molecules to undergo recombination, ____.

Accepted Answer

A)  ​they must be from the same species 
B)  ​their strands must separate as in replication 
C)  ​they must be cut and spliced at specific nucleotide sequences 
D)  ​one of the two DNA strands must be degraded 
E)  ​they must first be transcribed 
A)  ​they must be from the same species 
B)  ​their strands must separate as in replication 
C)  ​they must be cut and spliced at specific nucleotide sequences 
D)  ​one of the two DNA strands must be degraded 
E)  ​they must first be transcribed

Question 17

To answer the following questions, refer to the five items listed below:
-These are bacterial populations containing thousands or millions of identical copies of one to several genes.

Accepted Answer

A) restriction enzymes 
B) recombinant DNA 
C) plasmids 
D) clones 
E) restriction sites 
A) restriction enzymes 
B) recombinant DNA 
C) plasmids 
D) clones 
E) restriction sites

Question 18

Cross-breeding has been used to produce featherless chickens to ____.

Accepted Answer

A)  ​make it easier to process after slaughter 
B)  ​prevent a feather infecting disease 
C)  ​be more resistant to high temperatures in the desert 
D)  ​improve breeding efficiency 
E)  ​increase their lifespan 
A)  ​make it easier to process after slaughter 
B)  ​prevent a feather infecting disease 
C)  ​be more resistant to high temperatures in the desert 
D)  ​improve breeding efficiency 
E)  ​increase their lifespan

Question 19

What fraction of your DNA is the same as every other human being?

Accepted Answer

A)  ​50% 
B)  ​75% 
C)  ​90% 
D)  ​95% 
E)  ​99% 
A)  ​50% 
B)  ​75% 
C)  ​90% 
D)  ​95% 
E)  ​99%

Question 20

The polymerase chain reaction (PCR) uses ________, nucleotides, and a heat-resistant DNA polymerase to rapidly increase the number of molecules of a DNA fragment.

Accepted Answer

The answer of The polymerase chain reaction (PCR) uses ________,...

Exam 15: Studying and Manipulating Genomes

Why are cDNA libraries desirable for the expression of eukaryotic genes in prokaryotes?

Identifying an individual by his or her DNA is referred to as ____.

cDNA ____.

To answer the following questions, refer to the five items listed below: -The combination of a foreign piece of DNA with the DNA of another organism.

To answer the following questions refer to the four items listed below. -This is an enzyme used to produce cDNA.

Figure 15.2 ​ The following questionsrefer to the figure above illustrating the initial steps in the formation of recombinant DNA. -In panel 4, the base-paired DNA fragments are joined together with ____.

Figure 15.5 -In step 2 (above) in the identification of the colony that contains a targeted gene, ____.

Seed banks ____.

Restriction enzymes ____.

__________ can reveal the order of nucleotide bases in a fragment of DNA.

Genetic engineering has produced which of the following animals?​

Today's automated DNA sequencing machines can sequence an individual's entire genome in ____.

Small circular molecules of "extra" DNA in bacteria ____.

________, a viral enzyme, is used to convert single-stranded RNA into ___________ for cloning.

For the polymerase chain reaction to occur, ____.

In order for DNA molecules to undergo recombination, ____.

To answer the following questions, refer to the five items listed below: -These are bacterial populations containing thousands or millions of identical copies of one to several genes.

Cross-breeding has been used to produce featherless chickens to ____.

What fraction of your DNA is the same as every other human being?

The polymerase chain reaction (PCR) uses ________, nucleotides, and a heat-resistant DNA polymerase to rapidly increase the number of molecules of a DNA fragment.

Invitation to Biology

Lifes Chemical Basis

Molecules of Life

Cell Structure and Function

Ground Rules of Metabolism

Where It Starts - Photosynthesis

How Cells Release Chemical Energy

Dna Structure and Function

From Dna to Protein

Gene Control

How Cells Reproduce

Meiosis and Sexual Reproduction

Observing Patterns in Inherited Traits

Chromosomes and Human Inheritance

Evidence of Evolution

Processes of Evolution

Organizating Information About Species

Lifes Origin and Early Evolution

Viruses - Bacteria - and Archaea

Protists - the Simplest Eukaryotes

The Land Plants

Fungi

Animal Evolution - the Invertibrates

Animal Evolution - the Chordates

Human Evolution

Plant Tissues

Plant Nutrition and Transport

Life Cycles of Flowering Plants

Communication Strategies in Plants

Animal Tissues

Neural Control

Sensory Perception

Endocrine Control

Structural Support and Movement

Circulation

Immunity

Respiration

Digestion and Nutrition

Maintaining the Internal Environment

Animal Reproductive Systems

Animal Development

Animal Behavior

Filters

Figure 15.2 The following questionsrefer to the figure above illustrating the initial steps in the formation of recombinant DNA. -In panel 4, the base-paired DNA fragments are joined together with ____.

Genetic engineering has produced which of the following animals?

, a viral enzyme, is used to convert single-stranded RNA into ___ for cloning.