Question 1

RNA interference helps in

Accepted Answer

A) Cell proliferation 
B) Micropropagation 
C) Cell defence 
D) Cell differentiation 
A) Cell proliferation 
B) Micropropagation 
C) Cell defence 
D) Cell differentiation C

Question 2

Bacteria protect themselves from viruses by fragmenting viral DNA with

Accepted Answer

A) Ligase 
B) Endonuclease 
C) Exonuclease 
D) Gyrase 
A) Ligase 
B) Endonuclease 
C) Exonuclease 
D) Gyrase B

Question 3

ELISA is

Accepted Answer

A) Using radiolabelled second antibody 
B) Usage of RBCs 
C) Using complement-mediated cell lysis 
D) Addition of substrate that is converted into a coloured end product 
A) Using radiolabelled second antibody 
B) Usage of RBCs 
C) Using complement-mediated cell lysis 
D) Addition of substrate that is converted into a coloured end product D

Question 4

Southern blotting is

Accepted Answer

A) Attachment of probes to DNA fragments 
B) Transfer of DNA fragments from electrophoretic gel to a nitrocellulose sheet 
C) Comparison of DNA fragments to two sources 
D) Transfer of DNA fragments to electrophoretic gel from cellulose membrane 
A) Attachment of probes to DNA fragments 
B) Transfer of DNA fragments from electrophoretic gel to a nitrocellulose sheet 
C) Comparison of DNA fragments to two sources 
D) Transfer of DNA fragments to electrophoretic gel from cellulose membrane

Question 5

The Taq polymerase enzyme is obtained from

Accepted Answer

A) Thermus aquaticus 
B) Thiobacillus ferrooxidans 
C) Bacillus subtilis 
D) Pseudomonas subtilis 
A) Thermus aquaticus 
B) Thiobacillus ferrooxidans 
C) Bacillus subtilis 
D) Pseudomonas subtilis

Question 6

Which is a genetically modified crop?

Accepted Answer

A) Bt-cotton 
B) Bt-brinjal 
C) Golden rice 
D) All 
A) Bt-cotton 
B) Bt-brinjal 
C) Golden rice 
D) All

Question 7

The vaccines prepared through recombinant DNA technology are

Accepted Answer

A) Third generation vaccines 
B) First-generation vaccines 
C) Second-generation vaccines 
D) None 
A) Third generation vaccines 
B) First-generation vaccines 
C) Second-generation vaccines 
D) None

Question 8

A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using

Accepted Answer

A) Taq polymerase 
B) Polymerase III 
C) Ligase 
D) Eco RI 
A) Taq polymerase 
B) Polymerase III 
C) Ligase 
D) Eco RI

Question 9

Which of the following is not a component of downstream processing?

Accepted Answer

A) Expression 
B) Preservation 
C) Purification 
D) Separation 
A) Expression 
B) Preservation 
C) Purification 
D) Separation

Question 10

Excision and insertion of a gene is called

Accepted Answer

A) Biotechnology 
B) Genetic engineering 
C) Cytogenetics 
D) Gene therapy 
A) Biotechnology 
B) Genetic engineering 
C) Cytogenetics 
D) Gene therapy

Question 11

---- is used as a vector for cloning into higher organisms

Accepted Answer

A) Retrovirus 
B) Baculovirus 
C) Salmonella typhimurium 
D) Rhizopus nigricans 
A) Retrovirus 
B) Baculovirus 
C) Salmonella typhimurium 
D) Rhizopus nigricans

Question 12

Plasmids are used as cloning vectors for which of the following reasons?

Accepted Answer

A) Can be multiplied in culture 
B) Self-replication in bacterial cells 
C) Can be multiplied in laboratories with the help of enzymes 
D) Replicate freely outside bacterial cells 
A) Can be multiplied in culture 
B) Self-replication in bacterial cells 
C) Can be multiplied in laboratories with the help of enzymes 
D) Replicate freely outside bacterial cells

Question 13

Which of the following is an endonuclease?

Accepted Answer

A) DNase I 
B) Hind II 
C) Protease 
D) RNase restriction 
A) DNase I 
B) Hind II 
C) Protease 
D) RNase restriction

Question 14

Restriction enzymes were discovered by

Accepted Answer

A) Smith and Nathans 
B) Alexander Fleming 
C) Berg 
D) None 
A) Smith and Nathans 
B) Alexander Fleming 
C) Berg 
D) None

Question 15

The expression of a transgene in the target tissue is identified by a

Accepted Answer

A) Transgene 
B) Promoter 
C) Enhancer 
D) Reporter 
A) Transgene 
B) Promoter 
C) Enhancer 
D) Reporter

Question 16

The Golden Rice variety is rich in

Accepted Answer

A) Vitamin C 
B) ?-carotene and ferritin 
C) Biotin 
D) Lysine 
A) Vitamin C 
B) ?-carotene and ferritin 
C) Biotin 
D) Lysine

Question 17

The DNA fragments have sticky ends due to

Accepted Answer

A) Endonuclease 
B) Unpaired bases 
C) Calcium ions 
D) Free methylation 
A) Endonuclease 
B) Unpaired bases 
C) Calcium ions 
D) Free methylation

Question 18

The first transgenic plant to be produced is

Accepted Answer

A) Brinjal 
B) Tobacco 
C) Rice 
D) Cotton 
A) Brinjal 
B) Tobacco 
C) Rice 
D) Cotton

Question 19

Which of the following restriction enzymes produce blunt ends?

Accepted Answer

A) Hind III 
B) ho 
C) Eco RV 
D) Sal I 
A) Hind III 
B) ho 
C) Eco RV 
D) Sal I

Question 20

Klenow fragment is derived from

Accepted Answer

A) DNA Ligase 
B) DNA Pol-I 
C) DNA Pol-II 
D) Reverse Transcriptase 
A) DNA Ligase 
B) DNA Pol-I 
C) DNA Pol-II 
D) Reverse Transcriptase

RNA interference helps in

Bacteria protect themselves from viruses by fragmenting viral DNA with

ELISA is

Southern blotting is

The Taq polymerase enzyme is obtained from

Which is a genetically modified crop?

The vaccines prepared through recombinant DNA technology are

A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using

Which of the following is not a component of downstream processing?

Excision and insertion of a gene is called

---- is used as a vector for cloning into higher organisms

Plasmids are used as cloning vectors for which of the following reasons?

Which of the following is an endonuclease?

Restriction enzymes were discovered by

The expression of a transgene in the target tissue is identified by a

The Golden Rice variety is rich in

The DNA fragments have sticky ends due to

The first transgenic plant to be produced is

Which of the following restriction enzymes produce blunt ends?

Klenow fragment is derived from

Biotechnology and Animal Cell Culture

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Exam 1: Genetic Engineering and Biotechnology

RNA interference helps in

Bacteria protect themselves from viruses by fragmenting viral DNA with

ELISA is

Southern blotting is

The Taq polymerase enzyme is obtained from

Which is a genetically modified crop?

The vaccines prepared through recombinant DNA technology are

A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using

Which of the following is not a component of downstream processing?

Excision and insertion of a gene is called

---- is used as a vector for cloning into higher organisms

Plasmids are used as cloning vectors for which of the following reasons?

Which of the following is an endonuclease?

Restriction enzymes were discovered by

The expression of a transgene in the target tissue is identified by a

The Golden Rice variety is rich in

The DNA fragments have sticky ends due to

The first transgenic plant to be produced is

Which of the following restriction enzymes produce blunt ends?

Klenow fragment is derived from

Biotechnology and Animal Cell Culture

Filters