Exam 13: An Introduction to Genetic Technology

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Nuclear transfer is a preferred method of cloning over embryo splitting because it ________.

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C

Embryo splitting

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E

In DNA sequencing, the different sized fragments created are separated from each other by ____________________.

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gel electrophoresis

The number of copies of a DNA sequence produced by the polymerase chain reaction depends on the number of cycles of the reaction.

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A genetic library contains all the DNA fragments of

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Compared to the average cow's milk production, how many times greater was Blackrose II's milk production?

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Explain the technologies used in cloning domestic animals.

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What are the benefits of cloning animals?

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A fragment cut out of DNA and a plasmid cut open for insertion of the fragment must both have been cut with the same type of restriction enzyme.

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For what purpose do bacteria use restriction enzymes?

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In DNA recombinant technology, the enzyme that acts like glue is ______________.

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Cloning by embryo splitting

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In genomic libraries, DNA fragments are stored in ______________ chromosomes.

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DNA polymerase is used in the polymerase chain reaction.

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Plasmids are self-replicating single stranded fragments of eukaryotic DNA.

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In gel electrophoresis, what causes the DNA fragments to migrate?

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In recombinant DNA techniques, primers and DNA fragments are linked together by _________.

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The ends of DNA molecules cut by restriction enzymes are "sticky" because

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____________________ are short nucleotide sequences used to begin replication in PCR, and to which the polymerase adds nucleotides.

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Adult cells cannot be used as the source of nuclei for cloning animals.

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