Exam 5: Protein Purification and Characterization Techniques

Exhibit 5A The following diagram shows the anode, cathode, and pH gradient of an isoelectric focusing bed: Refer to Exhibit 5A. A mixture of asp, asn, and arg is placed in the bed where the pH is 7, and the current is turned on. From left to right, which best represents the final positions of the individual amino acids?

Which of the following explains why a secondary antibody is used in an ELISA?

In isoelectric focusing gel electrophoresis

If a protein with the sequence PQRKYPIG is treated with trypsin, what will the products be?

In an ELISA, the molecule that binds directly to the target protein is called the

In any form of chromatography, how will a compound which interacts more strongly with the stationary phase elute compared to one that interacts less strongly?

Which of the following is a similarity between ion-exchange chromatography and affinity chromatography?

Which of the following treatments results in a polypeptide fragment with a homoserine lactone at the C-terminal end?

In chromatography the experimental setup always requires

The process of breaking cells open to release the organelles is called _____.

In affinity chromatography, a protein

It is frequently possible to bypass the determination of the identity of the N-terminal amino acid of a protein because