Question 1

Which of the following is FALSE regarding regulation of gene expression in bacteria?

Accepted Answer

A)  Negative control of transcription involves the binding of a repressor protein to a regulatory DNA sequence, with the consequence of preventing transcription. 
B)  Repressor proteins commonly contain DNA- binding and allosteric domains. 
C)  There can be transcriptional regulation for the initiation and the amount of transcription, as well as post- transcriptional regulatory mechanisms. 
D)  Regulated transcription is necessary; constitutive transcription, continuously transcribing genes with no regulatory control, does not occur without mutation. 
E)  Positive control of transcription involves the binding of an activator protein to a regulatory DNA sequence, with the result of initiating transcription. 
A)  Negative control of transcription involves the binding of a repressor protein to a regulatory DNA sequence, with the consequence of preventing transcription. 
B)  Repressor proteins commonly contain DNA- binding and allosteric domains. 
C)  There can be transcriptional regulation for the initiation and the amount of transcription, as well as post- transcriptional regulatory mechanisms. 
D)  Regulated transcription is necessary; constitutive transcription, continuously transcribing genes with no regulatory control, does not occur without mutation. 
E)  Positive control of transcription involves the binding of an activator protein to a regulatory DNA sequence, with the result of initiating transcription.

Question 2

The enzyme fi- galactosidase catalyzes what reaction?

Accepted Answer

A)  lactose  →glucose + fructose 
B)  galactose  →glucose + lactose 
C)  allolactose  →glucose + lactose 
D)  lactose  →galactose + glucose 
E)  glucose  →galactose + lactose 
A)  lactose  →glucose + fructose 
B)  galactose  →glucose + lactose 
C)  allolactose  →glucose + lactose 
D)  lactose  →galactose + glucose 
E)  glucose  →galactose + lactose

Question 3

In negative control, what molecule would you expect to find bound to the operator if there is no transcription?

Accepted Answer

A)  inducer 
B)  activator 
C)  RNA polymerase 
D)  corepressor 
E)  repressor 
A)  inducer 
B)  activator 
C)  RNA polymerase 
D)  corepressor 
E)  repressor

Question 4

In E. coli, the cya gene encodes adenylate cyclase. Will cells that are cya- be able to grow on medium that contains lactose as the sole carbon source? Why or why not?

Accepted Answer

No. Metabolically, cells that are cya- w

Question 5

Which of the following genotypes and conditions will have the LOWEST level of trp operon expression?

Accepted Answer

A)  trpL- / tryptophan present 
B)  trp+ / tryptophan absent 
C)  trpP- / tryptophan absent 
D)  trpR- / tryptophan present 
E)  trp+ / tryptophan present 
A)  trpL- / tryptophan present 
B)  trp+ / tryptophan absent 
C)  trpP- / tryptophan absent 
D)  trpR- / tryptophan present 
E)  trp+ / tryptophan present

Question 6

Which enzyme is the product of the lacZ gene?

Accepted Answer

The answer of Which enzyme is the product of the...

Question 7

Which of these haploid strains produces β- galactosidase constitutively but does not produce permease?

Accepted Answer

A)  I+ P+ O- Z+ Y+ 
B)  I- P+ O+ Z+ Y- 
C)  I - P+ O+ Z+ Y+ 
D)  I+ P+ O+ Z- Y- 
E)  I- P+ O+ Z- Y+ 
A)  I+ P+ O- Z+ Y+ 
B)  I- P+ O+ Z+ Y- 
C)  I - P+ O+ Z+ Y+ 
D)  I+ P+ O+ Z- Y- 
E)  I- P+ O+ Z- Y+

Question 8

What would be the consequences to a h phage that lysogenized in a recA- bacterial cell? Explain why this would occur.

Accepted Answer

Induction (the termination of lysogeny a

Question 9

The genetic switch controlling whether a bacterium enters the lytic or lysogenic cycle relies on the binding of which two proteins?

Accepted Answer

The answer of The genetic switch controlling whether a bacterium...

Question 10

In the lac operon, what acts as the inducer?

Accepted Answer

A)  glucose 
B)  transacetylase 
C)  permease 
D)  allolactose 
E)  β- galactosidase 
A)  glucose 
B)  transacetylase 
C)  permease 
D)  allolactose 
E)  β- galactosidase

Question 11

Which of the following is FALSE regarding translational regulation in bacteria?

Accepted Answer

A)  It is the most predominant mode of controlling gene expression in bacteria. 
B)  Translation repressor proteins regulate translation by binding mRNA near the Shine- Dalgarno sequence. 
C)  The bacterial insertion sequence (IS10) uses antisense RNA to regulate translation of the mRNA that produces the enzyme transposase. 
D)  Antisense RNA can bind to a portion of a specific mRNA to which it is complementary. 
E)  The binding of an mRNA by an antisense RNA can prevent ribosome attachment to the mRNA and block translation. 
A)  It is the most predominant mode of controlling gene expression in bacteria. 
B)  Translation repressor proteins regulate translation by binding mRNA near the Shine- Dalgarno sequence. 
C)  The bacterial insertion sequence (IS10) uses antisense RNA to regulate translation of the mRNA that produces the enzyme transposase. 
D)  Antisense RNA can bind to a portion of a specific mRNA to which it is complementary. 
E)  The binding of an mRNA by an antisense RNA can prevent ribosome attachment to the mRNA and block translation.

Question 12

Which region of the h phage genome enables the linear chromosome to circularize when it enters a host cell?

Accepted Answer

A)  cohesive (cos) site 
B)  early operators 
C)  integrase (int) gene 
D)  IS10 
E)  excisionase (xis) gene 
A)  cohesive (cos) site 
B)  early operators 
C)  integrase (int) gene 
D)  IS10 
E)  excisionase (xis) gene

Question 13

Expression of a(n)_____________ alters gene transcription in E. coli by activating transcription of specialized heat stress response genes.

Accepted Answer

alternativ

Question 14

DNA- binding proteins exert control by binding DNA at specific sequences. Describe how DNA and protein interact at the molecular level. Are proteins able to identify specific DNA sequences, or do they bind to all regions with equal frequency? What motifs are commonly found in DNA- binding proteins?

Accepted Answer

DNA- protein interactions occur when the

Question 15

For each of the following E. coli partial diploid strains, indicate whether expression of fi- galactosidase and permease is inducible (I), constitutive (C), or noninducible (N). Assume glucose levels are very low. Which of the strains are able to use lactose as their sole carbon source?
 $$\begin{array} { | l | l | l | l | } 
\hline && \beta \text {-galactosidase } & \text { Permease } \
\hline \text { a. } & I ^ { s } O ^ { + } Z Y ^ { + } / I ^ { + } O ^ { C } Z ^ { + } Y & & \
\hline \text { b. } & I ^ { + } O ^ { + } Z Y ^ { + } / I ^ { + } P ^ { - } O ^ { + } Z ^ { + } Y ^ { + } & & \
\hline \text { c. } & I ^ { + } O ^ { C } Z Y ^ { + } / \Gamma O ^ { + } Z ^ { + } Y ^ { + } & & \
\hline \text { d. } & I ^ { + } O ^ { + } Z ^ { + } Y / I ^ { + } P ^ { - } O ^ { C } Z ^ { + } Y ^ { + } & & \
\hline
\end{array}$$

Accepted Answer

@#IMG-DLM& Only strain c is able to use

Question 16

Which of the following genotypes and conditions will have the HIGHEST level of trp operon expression?

Accepted Answer

A)  trpL- / tryptophan present 
B)  trp+ / tryptophan absent 
C)  trpR- / tryptophan present 
D)  trp+ / tryptophan present 
E)  trpP- / tryptophan absent 
A)  trpL- / tryptophan present 
B)  trp+ / tryptophan absent 
C)  trpR- / tryptophan present 
D)  trp+ / tryptophan present 
E)  trpP- / tryptophan absent

Question 17

Which of the following can be mutated to result in constitutive expression from the lac operon?

Accepted Answer

A)  lacO and lacI 
B)  lacO and lacZ 
C)  lacI and lacY 
D)  lacZ and lacI 
E)  lacZ and lacP 
A)  lacO and lacI 
B)  lacO and lacZ 
C)  lacI and lacY 
D)  lacZ and lacI 
E)  lacZ and lacP

Question 18

When both glucose and lactose are available in the growth medium, the presence of which molecule is capable of inducing basal transcription of the lac operon?

Accepted Answer

The answer of When both glucose and lactose are available...

Question 19

Which of the following is FALSE regarding how bacteria regulate the transcription of stress response genes?

Accepted Answer

A)  High heat can cause a change in the chaperone proteins. 
B)  The rpoH gene encodes a sigma factor known as a32 that is active at high temperatures. 
C)  Holoenzymes containing an alternative sigma subunit are able to recognize the promoters of genes not transcribed by the common bacterial RNA polymerase. 
D)  Under extreme growth conditions, such as heat stress and starvation, bacteria can induce transcription of alternative sigma factors. 
E)  Transcription is activated by antitermination, which allows transcription to extend into heat shock genes. 
A)  High heat can cause a change in the chaperone proteins. 
B)  The rpoH gene encodes a sigma factor known as a32 that is active at high temperatures. 
C)  Holoenzymes containing an alternative sigma subunit are able to recognize the promoters of genes not transcribed by the common bacterial RNA polymerase. 
D)  Under extreme growth conditions, such as heat stress and starvation, bacteria can induce transcription of alternative sigma factors. 
E)  Transcription is activated by antitermination, which allows transcription to extend into heat shock genes.

Question 20

Which molecule functions as the corepressor in the trp operon?

Accepted Answer

The answer of Which molecule functions as the corepressor in...

Which of the following is FALSE regarding regulation of gene expression in bacteria?

The enzyme fi- galactosidase catalyzes what reaction?

In negative control, what molecule would you expect to find bound to the operator if there is no transcription?

In E. coli, the cya gene encodes adenylate cyclase. Will cells that are cya- be able to grow on medium that contains lactose as the sole carbon source? Why or why not?

Which of the following genotypes and conditions will have the LOWEST level of trp operon expression?

Which enzyme is the product of the lacZ gene?

Which of these haploid strains produces β- galactosidase constitutively but does not produce permease?

What would be the consequences to a h phage that lysogenized in a recA- bacterial cell? Explain why this would occur.

The genetic switch controlling whether a bacterium enters the lytic or lysogenic cycle relies on the binding of which two proteins?

In the lac operon, what acts as the inducer?

Which of the following is FALSE regarding translational regulation in bacteria?

Which region of the h phage genome enables the linear chromosome to circularize when it enters a host cell?

Expression of a(n)_____________ alters gene transcription in E. coli by activating transcription of specialized heat stress response genes.

DNA- binding proteins exert control by binding DNA at specific sequences. Describe how DNA and protein interact at the molecular level. Are proteins able to identify specific DNA sequences, or do they bind to all regions with equal frequency? What motifs are commonly found in DNA- binding proteins?

Which of the following genotypes and conditions will have the HIGHEST level of trp operon expression?

Which of the following can be mutated to result in constitutive expression from the lac operon?

When both glucose and lactose are available in the growth medium, the presence of which molecule is capable of inducing basal transcription of the lac operon?

Which of the following is FALSE regarding how bacteria regulate the transcription of stress response genes?

Which molecule functions as the corepressor in the trp operon?

The Molecular Basis of Heredity, Variation, and Evolution

Transmission Genetics

Cell Division and Chromosome Heredity

Inheritance Patterns of Single Genes and Gene Interaction

Genetic Linkage and Mapping in Eukaryotes

Genetic Analysis and Mapping in Bacteria and Bacteriophage

Dna Structure and Replication

Molecular Biology of Transcription and Rna Processing

The Molecular Biology of Translation

Chromosome Chapter Title Tbd

Gene Mutation, Dna Repair, and Homologous Recombination

Regulation of Gene Expression in Bacteria and Bacteriophage

Regulation of Gene Expression in Eukaryotes

Analysis of Gene Function by Forward Genetics and Reverse Genetics

Recombinant Dna Technology and Its Applications

Genomics: Genetics From a Whole-Genome Perspective

Organelle Inheritance and the Evolution of Organelle Genomes

Developmental Genetics

Genetic Analysis of Quantitative Traits

Population Genetics and Evolution at the Population, Species, and Molecular Levels

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Exam 12: Chromosome Exam Title Tbd

Which of the following is FALSE regarding regulation of gene expression in bacteria?

The enzyme fi- galactosidase catalyzes what reaction?

In negative control, what molecule would you expect to find bound to the operator if there is no transcription?

In E. coli, the cya gene encodes adenylate cyclase. Will cells that are cya- be able to grow on medium that contains lactose as the sole carbon source? Why or why not?

Which of the following genotypes and conditions will have the LOWEST level of trp operon expression?

Which enzyme is the product of the lacZ gene?

Which of these haploid strains produces β- galactosidase constitutively but does not produce permease?

What would be the consequences to a h phage that lysogenized in a recA- bacterial cell? Explain why this would occur.

The genetic switch controlling whether a bacterium enters the lytic or lysogenic cycle relies on the binding of which two proteins?

In the lac operon, what acts as the inducer?

Which of the following is FALSE regarding translational regulation in bacteria?

Which region of the h phage genome enables the linear chromosome to circularize when it enters a host cell?

Expression of a(n)_____________ alters gene transcription in E. coli by activating transcription of specialized heat stress response genes.

DNA- binding proteins exert control by binding DNA at specific sequences. Describe how DNA and protein interact at the molecular level. Are proteins able to identify specific DNA sequences, or do they bind to all regions with equal frequency? What motifs are commonly found in DNA- binding proteins?

Which of the following genotypes and conditions will have the HIGHEST level of trp operon expression?

Which of the following can be mutated to result in constitutive expression from the lac operon?

When both glucose and lactose are available in the growth medium, the presence of which molecule is capable of inducing basal transcription of the lac operon?

Which of the following is FALSE regarding how bacteria regulate the transcription of stress response genes?

Which molecule functions as the corepressor in the trp operon?

The Molecular Basis of Heredity, Variation, and Evolution

Transmission Genetics

Cell Division and Chromosome Heredity

Inheritance Patterns of Single Genes and Gene Interaction

Genetic Linkage and Mapping in Eukaryotes

Genetic Analysis and Mapping in Bacteria and Bacteriophage

Dna Structure and Replication

Molecular Biology of Transcription and Rna Processing

The Molecular Biology of Translation

Chromosome Chapter Title Tbd

Gene Mutation, Dna Repair, and Homologous Recombination

Regulation of Gene Expression in Bacteria and Bacteriophage

Regulation of Gene Expression in Eukaryotes

Analysis of Gene Function by Forward Genetics and Reverse Genetics

Recombinant Dna Technology and Its Applications

Genomics: Genetics From a Whole-Genome Perspective

Organelle Inheritance and the Evolution of Organelle Genomes

Developmental Genetics

Genetic Analysis of Quantitative Traits

Population Genetics and Evolution at the Population, Species, and Molecular Levels

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