Exam 11: Analyzing Genomic Variation

In 2008, Margaret Binkele was murdered in her Georgia home.The number of repeats at three CODIS simple sequence repeat (SSR)loci were determined using crime scene samples and blood from three suspects.The results are summarized in the table. Genotypes at three SSR loci Crime scerie sarmple Suspect 1 Suspect 2 Suspect 3 SSR locus 1 10,10 8 ,10 6,7 10,10 SSR locus 2 11,11 11,11 9,15 10,12 SSR locus 3 6,9 6,7 6,9 6,9 -According to the data, the crime scene sample matches the sample from which suspect?

What additional data would increase your confidence that the gene of interest and the DNA marker are linked? (Select all that apply. )

In the United States, most newborns undergo a screening test for up to 60 genetic disorders.A few drops of blood is taken from each baby and the levels of various blood components are determined.The screening identifies babies who might have a genetic disorder, but is not diagnostic.Further testing is required if the screen shows a blood component is out of the normal range.Which of the following technologies could potentially be a low-cost replacement for the current newborn screening and would directly test most babies for the presence of many genetic disorders?

Simple sequence repeat loci are highly variable because of what process?

If a single DNA molecule is amplified by PCR, how many DNA molecules will exist after FIVE cycles?

Which is a challenge of using pedigrees for positional cloning?

Simple sequence repeats are

Fragile X syndrome is caused by expansion of a trinucleotide repeat in the 5′ UTR of the FMR-1 gene.What is the simplest way to detect this expansion?

Which is the most common type of DNA polymorphism?

Which is part of a DNA sequencing technique that is useful for high-throughput sequencing, but is not part of Sanger DNA sequencing?

Given that an individual is a compound heterozygote at the CFTR locus, the gene that causes cystic fibrosis, what can be inferred?

What is the name of the process for removing amniotic fluid from a pregnant mother to obtain fetal cells for genetic testing?

Herceptin is a drug that is given to treat certain breast cancers.However, it is most effective on tumors that are overexpressing the gene HER2.Which of the following strategies would be best for determining whether Herceptin would be effective in a given patient?

What mechanisms contribute to the formation of deletions and insertions? (Select all that apply. )

You are studying a disease that is known to be caused by a single nucleotide change in a single gene, although the effect this change ultimately has on the protein's structure and function is unknown.You have DNA samples from multiple patients that you suspect of having this disease.What is the most efficient way to test the samples for the relevant mutation?

Linkage between a gene of interest and a DNA marker is being studied. A mating results in 6 parental and 3 recombinant offspring -The results of this mating are sufficient to conclude with confidence that the gene of interest and the DNA marker are linked.

If the 13 CODIS SSR loci are genotyped, why does each person (except identical twins)have a unique combination of SSR alleles?

How can microarrays differentiate between a wild-type allele and a disease allele that differ at only one nucleotide?