What Is a Significant Problem with Regard to Making CDNA

What is a significant problem with regard to making cDNA libraries to analyze overall gene function in an eukaryotic organism?

A) A cDNA library will only contain genes that are actively transcribed in the organism.
B) Reverse transcriptase cannot be used to make cDNA libraries.
C) cDNA cannot be amplified by PCR.
D) cDNA libraries do not contain intron sequences.
E) cDNA libraries may contain several alternatively spliced forms of the same gene.

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