Which Observation Suggested to Sutherland the Involvement of a Second

Which observation suggested to Sutherland the involvement of a second messenger in adrenalineadrenaline's effect on liver cells?

A) Enzymatic activity was proportional to the amount of calcium added to a cell-free extract.
B) Receptor studies indicated that adrenaline adrenalinewas a ligand.
C) Glycogen breakdown was observed only when adrenaline adrenalinewas administered to intact cells.
D) Glycogen breakdown was observed only when adrenaline adrenalineand glycogen phosphorylase were mixed.

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