Which Observation Suggested to Sutherland (1971 Nobel Prize Winner) the Involvement

Which observation suggested to Sutherland (1971 Nobel Prize winner) the involvement of a second messenger in adrenaline's effect on liver cells?

A) Receptor studies indicated that adrenaline was a ligand.
B) Glycogen breakdown was observed only when adrenaline was administered to intact cells.
C) Glycogen breakdown was observed when adrenaline and glycogen phosphorylase were combined in a cell-free system.
D) Adrenaline was known to have different effects on many types of cells.

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