What External Stimulation Is Required by Mammalian Cells to Progress

What external stimulation is required by mammalian cells to progress through the cycle after they have passed the restriction point?

A) the presence of steroid hormones in their culture medium
B) the presence of Na⁺ ions in their culture medium
C) the presence of growth factors in their culture medium
D) no external stimulation is required after the restriction point is passed
E) the presence of vitamins in their culture medium

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