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Insulin Is a Polypeptide Hormone That Contains Two Short Polypeptide

Question 7

Multiple Choice

Insulin is a polypeptide hormone that contains two short polypeptide chains linked by two interstrand disulfide bonds. The most logical order of events to perform in order to sequence this protein would be:
A.The peptides are reduced with mercaptoethanol.
B.The peptides are sequenced using Edman chemistry.
C.The peptides are separated by chromatography techniques.
D.The peptides are alkylated with iodoacetamide.


A) A, D, C, B
B) C, A, D, B
C) C, B, A, D
D) A, B, C, D
E) A, C, D, B

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