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Glyceraldehyde 3-Phosphate Dehydrogenase Catalyzes the Reaction Between Glyceraldehyde 3-Phosphate, NAD+

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Glyceraldehyde 3-phosphate dehydrogenase catalyzes the reaction between glyceraldehyde 3-phosphate, NAD+, and phosphate ion to form 1,3-bisphosphoglycerate and NADH.Under certain conditions, a permanently radiolabeled enzyme intermediate forms if 14C-glyceraldehyde 3-phosphate and NAD+ are used as substrates and phosphate is left out of the reaction mix.The radiolabeled molecule is linked to a cysteine side chain of the enzyme.This result suggests that this enzyme uses _______ as a catalytic mechanism.

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