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A Graduate Student at SDSU Wants to Measure the Activity

Question 31

Multiple Choice

A graduate student at SDSU wants to measure the activity of a particular enzyme at pH 4.0.To buffer her reaction,she will use a buffer system based on one of the acids listed below,which acid is most appropriate for the experiment?


A) Formic acid (Ka 1.78 × 10−4)
B) MES (Ka 8.13 × 10−7)
C) PIPES (Ka 1.74 × 10−7)
D) Tris (Ka 8.32 × 10−9)
E) Piperidine (Ka 7.58 × 10−12)

Correct Answer:

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