____________________ Is Used to Isolate a Single Compound or Class

____________________ is used to isolate a single compound or class of compounds from a complex mixture by passing the sample through a column where only one solute is bound.The remaining solutes pass through the column.The bound solute is eluted from the column by changing a condition such as________________________________________ to weaken its binding.

A) Adhesion chromatography;ratio of polar and nonpolar solvents
B) Binding chromatography;pH or ionic strength
C) Affinity chromatography;the polarity of the solvent
D) Adhesion chromatography;the temperature of the column
E) Affinity chromatography;pH or ionic strength

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