Gradient Elution Is Used in HPLC

Gradient elution is used in HPLC.How is the gradient created for a reverse-phase HPLC experiment?

A) Increase the pH of the mobile phase over time by adding strong base.
B) Decrease the pH of the mobile phase over time by adding strong acid.
C) Increase the vapor pressure of the analytes over time by increasing the temperature.
D) Increase the polarity of the mobile phase over time by mixing with a more polar solvent.
E) Decrease the polarity of the mobile phase over time by mixing with a less polar solvent.

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