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Nested Polymerase Chain Reaction (PCR)is Very Sensitive and Specific Because

Question 27

Multiple Choice

Nested polymerase chain reaction (PCR) is very sensitive and specific because:


A) It uses more than one DNA polymerase to make three to four times the amount of PCR products than standard PCR.
B) The assay itself basically serves as a form of internal control and ensures specificity.
C) The assay will act as a form of external control, ensuring specificity and sensitivity.
D) The fluorochrome that is attached to the primers ensures specificity of the nucleotides that are attached to the template.

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