Nested Polymerase Chain Reaction (PCR) Is Very Sensitive and Specific

Nested polymerase chain reaction (PCR) is very sensitive and specific because:

A) it uses more than one DNA polymerase to make three to four times the amount of PCR products than standard PCR.
B) the assay itself basically serves as a form of internal control and ensures specificity.
C) the assay will act as a form of external control, ensuring specificity and sensitivity.
D) the fluorochrome that is attached to the primers ensures specificity of the nucleotides that are attached to the template.

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