What Is the Most Logical Sequence of Steps for Splicing

What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
I. Transform bacteria with recombinant DNA molecule.
II. Cut the plasmid DNA using restriction enzymes.
III. Extract plasmid DNA from bacterial cells.
IV. Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.
V. Use ligase to seal plasmid DNA to nonplasmid DNA.

A) I, II, IV, III, V
B) II, III, V, IV, I
C) III, II, IV, V, I
D) III, IV, V, I, II
E) IV, V, I, II, III

Correct Answer:

Verified

Unlock this answer now
Get Access to more Verified Answers free of charge

Access For Free