A linear piece of viral DNA of 8 kb can be cut with either of two restriction enzymes (X and Y). These are subjected to electrophoresis and produce the following bands:

\begin{array}{llcc} \text { X } & \text { Y } \\\underline{\quad\quad}&&5.0\\&\underline{\quad\quad}&4.5\\\underline{\quad\quad}&&3.0\\&\underline{\quad\quad} &2.5\\&\underline{\quad\quad} &1.0\\\end{array}

Cutting the same 8 kb piece with both enzymes together results in bands at 4.0, 2.5, 1.0, and 0.5.
Of the possible arrangements of the sites given below, which one is most likely?
A)
$A linear piece of viral DNA of 8 kb can be cut with either of two restriction enzymes (X and Y). These are subjected to electrophoresis and produce the following bands: \begin{array}{llcc} \text { X } & \text { Y } \\ \underline{\quad\quad}&&5.0\\ &\underline{\quad\quad}&4.5\\ \underline{\quad\quad}&&3.0\\ &\underline{\quad\quad} &2.5\\ &\underline{\quad\quad} &1.0\\ \end{array} Cutting the same 8 kb piece with both enzymes together results in bands at 4.0, 2.5, 1.0, and 0.5. Of the possible arrangements of the sites given below, which one is most likely? A) B) C) D) E)$
B)
$A linear piece of viral DNA of 8 kb can be cut with either of two restriction enzymes (X and Y). These are subjected to electrophoresis and produce the following bands: \begin{array}{llcc} \text { X } & \text { Y } \\ \underline{\quad\quad}&&5.0\\ &\underline{\quad\quad}&4.5\\ \underline{\quad\quad}&&3.0\\ &\underline{\quad\quad} &2.5\\ &\underline{\quad\quad} &1.0\\ \end{array} Cutting the same 8 kb piece with both enzymes together results in bands at 4.0, 2.5, 1.0, and 0.5. Of the possible arrangements of the sites given below, which one is most likely? A) B) C) D) E)$
C)
$A linear piece of viral DNA of 8 kb can be cut with either of two restriction enzymes (X and Y). These are subjected to electrophoresis and produce the following bands: \begin{array}{llcc} \text { X } & \text { Y } \\ \underline{\quad\quad}&&5.0\\ &\underline{\quad\quad}&4.5\\ \underline{\quad\quad}&&3.0\\ &\underline{\quad\quad} &2.5\\ &\underline{\quad\quad} &1.0\\ \end{array} Cutting the same 8 kb piece with both enzymes together results in bands at 4.0, 2.5, 1.0, and 0.5. Of the possible arrangements of the sites given below, which one is most likely? A) B) C) D) E)$
D)
$A linear piece of viral DNA of 8 kb can be cut with either of two restriction enzymes (X and Y). These are subjected to electrophoresis and produce the following bands: \begin{array}{llcc} \text { X } & \text { Y } \\ \underline{\quad\quad}&&5.0\\ &\underline{\quad\quad}&4.5\\ \underline{\quad\quad}&&3.0\\ &\underline{\quad\quad} &2.5\\ &\underline{\quad\quad} &1.0\\ \end{array} Cutting the same 8 kb piece with both enzymes together results in bands at 4.0, 2.5, 1.0, and 0.5. Of the possible arrangements of the sites given below, which one is most likely? A) B) C) D) E)$
E)
$A linear piece of viral DNA of 8 kb can be cut with either of two restriction enzymes (X and Y). These are subjected to electrophoresis and produce the following bands: \begin{array}{llcc} \text { X } & \text { Y } \\ \underline{\quad\quad}&&5.0\\ &\underline{\quad\quad}&4.5\\ \underline{\quad\quad}&&3.0\\ &\underline{\quad\quad} &2.5\\ &\underline{\quad\quad} &1.0\\ \end{array} Cutting the same 8 kb piece with both enzymes together results in bands at 4.0, 2.5, 1.0, and 0.5. Of the possible arrangements of the sites given below, which one is most likely? A) B) C) D) E)$

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