Performance of the "Factor Requirement Test" for Haemophilus Involves

Performance of the "factor requirement test" for Haemophilus involves

A) inoculation of unsupplemented media with a light suspension of the organism and placement of X and V factor disks on the agar surface.
B) inoculation of liquid media, unsupplemented and supplemented with X and V factors.
C) detecting the presence of enzymes that convert d-aminolevulinic acid (ALA) into porphyrins.
D) growth of the organism in the presence of bacterial species that produce X and V factors as metabolic by-products.

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