In an ELISA Test (Enzyme-Linked Immunosorbent Assay)what Is the Reason

In an ELISA test (enzyme-linked immunosorbent assay) what is the reason alkaline phosphatase is linked to the secondary antibody?

A) It ensures that contaminates from the medium are removed.
B) It can cause a color change to occur,indicating the presence of the specified antigen.
C) This enzyme is necessary to recognize the MHC found on infected cells.
D) All of the above answers apply.
E) Answers A and B both apply.

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