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The Transcription of a Gene In-Vitro Is Activated by Protein

Question 23

Multiple Choice

The transcription of a gene in-vitro is activated by protein A. This activation is repressed when protein B is present which is coded for on a different chromosome. Before protein B is added, protein A can be isolated from the reaction mixture, but when protein B is also present, only a much larger protein is able to be isolated. What is the explanation for this observation?


A) The cis-acting protein B is a repressor that binds to protein A, preventing it from binding to the activation sequence.
B) The cis-acting protein B is binding to the activation sequence, preventing protein A from being able to access and bind to the target sequence.
C) Chromatin remodelling is preventing protein A and/or B from binding to the activation sequence.
D) The trans-acting protein B is a repressor that binds to protein A, preventing it from binding to the activation sequence.
E) The trans-acting protein B is binding to the activation sequence, preventing protein A from being able to access and bind to the target sequence.

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