A Number of Seminal Experiments by Plant Pathologists on Tobacco

A number of seminal experiments by plant pathologists on tobacco infected with tobacco mosaic virus (TMV) established the principle of the existence of viruses. Which of the following were NOT techniques used to help establish the existence of viruses?

A) Precipitation of infective agents with ethanol that retained infectivity
B) Culturing of small particles
C) The ability of infective agents to be passed through a porcelain filter with pores too small to permit bacteria to pass through them
D) Sap from diseased plants could infect non-diseased plants
E) Diffusion of infective agents from sap into a block of gelatin

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