A Scientist Wants to Produce a Large Quantity of a Pectinase

A scientist wants to produce a large quantity of a pectinase enzyme, which breaks down cell walls and has various industrial applications. The scientist has also identified a yeast that produces and secretes this enzyme. How would she use this fungus to produce the pectinase in industrially significant concentrations in a short time frame?

A) Grow the fungus in a fermentation vat to high numbers and purify the enzyme from the media.
B) Grow the yeast on solid media until there is a thick culture, then purify the enzyme from the media.
C) Grow the fungus in shake flasks and purify the enzyme from the media.
D) Genetically engineer the yeast to produce very high amounts of the pectinase, then grow the culture.
E) Grow several generations of the yeast, measuring pectinase output at each generation, and select for those with the highest output.

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