Multiple Choice
Subtilisin is a bacterial serine protease that is commonly used as an additive in laundry detergent. In its native state, it has a methionine that becomes oxidized, surprisingly leading to enzyme inactivation. To prevent inactivation, subtilisin could be engineered by mutating methionine residues to ___________.
I. isoleucine
II. alanine
III. cysteine
IV. phenylalanine
A) I
B) II
C) III
D) II, III and IV
E) I, II and IV
Correct Answer:
Verified
Correct Answer:
Verified
Q1: _ of protein structures is a useful
Q3: Proteins can be engineered to express a
Q4: In the cell, most proteins fold without
Q5: It is common, when making mutations in
Q6: Using a resin in solid-phase protein synthesis
Q7: Several neurodegenerative diseases (Alzheimer's, Parkinson's, etc.) present
Q8: Mammalian protein expression systems _.<br>I. sometimes use
Q9: Which amino acid side chains would need
Q10: PCR-based mutagenesis is a widely used technique
Q11: Expression vectors are engineered fragments of DNA