A Scientist Is Investigating the Pattern of Gene Expression

A scientist is investigating the pattern of gene expression. Messenger RNA is extracted from various tissues, electrophoresed, blotted, and probed with radiolabeled DNA containing exon 4 sequences from that gene. An x-ray film is then put over the blotting membrane, and the autoradiogram findings are presented below: Which of the following best explains the autoradiogram findings in the different tissues?

A) Alternate RNA splicing
B) DNA rearrangement
C) DNA mutation
D) Enhancer effect
E) Transcription factor effect

Correct Answer:

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