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Passage Umami Is One of Five Basic Tastes That Humans Recognize

Question 53

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Passage
Umami is one of five basic tastes that humans recognize, and it is triggered by L-amino acids as well as some synthetic ingredients and organic acids.  Three common umami tastant molecules are shown in Figure 1.
Passage Umami is one of five basic tastes that humans recognize, and it is triggered by L-amino acids as well as some synthetic ingredients and organic acids.  Three common umami tastant molecules are shown in Figure 1. <strong>Figure 1</strong>  Three common tastant molecules known to trigger the umami taste sensationUmami taste signals are triggered upon binding of tastant molecules to the G protein-coupled receptor (GPCR) heterodimer T1R1-T1R3, which ultimately causes the breakdown of phosphatidylinositol 4,5-bisphosphate (PIP<sub>2</sub>) into the messenger molecules inositol-1,4,5-trisphosphate (IP<sub>3</sub>) and diacylglycerol (DAG) .  IP<sub>3</sub> triggers the release of Ca<sup>2+</sup> cations and the subsequent gating of the taste-transduction channels (Figure 2) . <strong>Figure 2</strong>  Signal pathway for transduction of umami taste sensationInosine monophosphate (IMP) has been suggested as enhancing (potentiating) the response of T1R1+T1R3 GPCRs to umami tastant molecules.  Scientists tested this hypothesis by culturing receptors from human embryonic kidney (HEK) cells containing promiscuous G proteins.  The HEK cells were then stimulated with umami tastants in the presence or absence of IMP.  In each test, the cells were exposed to the same amounts of amino acid tastant and IMP.  The number of cells that responded to each tastant was measured using a calcium indicator dye (FURA-2AM) in conjunction with fluorescence microscopy.  By comparing the cellular response ratio of the stimulated to unstimulated HEK cells, the extent of potentiation can be assessed.  The results are shown in Figure 3. <strong>Figure 3</strong>  Quantification of amino-acid responses for T1R1+T1R3 with and without IMP Adapted from Nelson G, Chandrashekar J, Hoon MA, et al. An amino-acid taste receptor. Nature. 2002;416(6877) :199-202. -Which of the following statements is true concerning the results shown in Figure 3? A) For the compounds potentiated by IMP, the extent of potentiation is five-fold. B) For the compounds potentiated by IMP, the extent of potentiation is equal. C) IMP most effectively potentiates Thr. D) IMP most effectively potentiates Asp. Figure 1  Three common tastant molecules known to trigger the umami taste sensationUmami taste signals are triggered upon binding of tastant molecules to the G protein-coupled receptor (GPCR) heterodimer T1R1-T1R3, which ultimately causes the breakdown of phosphatidylinositol 4,5-bisphosphate (PIP2) into the messenger molecules inositol-1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) .  IP3 triggers the release of Ca2+ cations and the subsequent gating of the taste-transduction channels (Figure 2) .
Passage Umami is one of five basic tastes that humans recognize, and it is triggered by L-amino acids as well as some synthetic ingredients and organic acids.  Three common umami tastant molecules are shown in Figure 1. <strong>Figure 1</strong>  Three common tastant molecules known to trigger the umami taste sensationUmami taste signals are triggered upon binding of tastant molecules to the G protein-coupled receptor (GPCR) heterodimer T1R1-T1R3, which ultimately causes the breakdown of phosphatidylinositol 4,5-bisphosphate (PIP<sub>2</sub>) into the messenger molecules inositol-1,4,5-trisphosphate (IP<sub>3</sub>) and diacylglycerol (DAG) .  IP<sub>3</sub> triggers the release of Ca<sup>2+</sup> cations and the subsequent gating of the taste-transduction channels (Figure 2) . <strong>Figure 2</strong>  Signal pathway for transduction of umami taste sensationInosine monophosphate (IMP) has been suggested as enhancing (potentiating) the response of T1R1+T1R3 GPCRs to umami tastant molecules.  Scientists tested this hypothesis by culturing receptors from human embryonic kidney (HEK) cells containing promiscuous G proteins.  The HEK cells were then stimulated with umami tastants in the presence or absence of IMP.  In each test, the cells were exposed to the same amounts of amino acid tastant and IMP.  The number of cells that responded to each tastant was measured using a calcium indicator dye (FURA-2AM) in conjunction with fluorescence microscopy.  By comparing the cellular response ratio of the stimulated to unstimulated HEK cells, the extent of potentiation can be assessed.  The results are shown in Figure 3. <strong>Figure 3</strong>  Quantification of amino-acid responses for T1R1+T1R3 with and without IMP Adapted from Nelson G, Chandrashekar J, Hoon MA, et al. An amino-acid taste receptor. Nature. 2002;416(6877) :199-202. -Which of the following statements is true concerning the results shown in Figure 3? A) For the compounds potentiated by IMP, the extent of potentiation is five-fold. B) For the compounds potentiated by IMP, the extent of potentiation is equal. C) IMP most effectively potentiates Thr. D) IMP most effectively potentiates Asp. Figure 2  Signal pathway for transduction of umami taste sensationInosine monophosphate (IMP) has been suggested as enhancing (potentiating) the response of T1R1+T1R3 GPCRs to umami tastant molecules.  Scientists tested this hypothesis by culturing receptors from human embryonic kidney (HEK) cells containing promiscuous G proteins.  The HEK cells were then stimulated with umami tastants in the presence or absence of IMP.  In each test, the cells were exposed to the same amounts of amino acid tastant and IMP.  The number of cells that responded to each tastant was measured using a calcium indicator dye (FURA-2AM) in conjunction with fluorescence microscopy.  By comparing the cellular response ratio of the stimulated to unstimulated HEK cells, the extent of potentiation can be assessed.  The results are shown in Figure 3.
Passage Umami is one of five basic tastes that humans recognize, and it is triggered by L-amino acids as well as some synthetic ingredients and organic acids.  Three common umami tastant molecules are shown in Figure 1. <strong>Figure 1</strong>  Three common tastant molecules known to trigger the umami taste sensationUmami taste signals are triggered upon binding of tastant molecules to the G protein-coupled receptor (GPCR) heterodimer T1R1-T1R3, which ultimately causes the breakdown of phosphatidylinositol 4,5-bisphosphate (PIP<sub>2</sub>) into the messenger molecules inositol-1,4,5-trisphosphate (IP<sub>3</sub>) and diacylglycerol (DAG) .  IP<sub>3</sub> triggers the release of Ca<sup>2+</sup> cations and the subsequent gating of the taste-transduction channels (Figure 2) . <strong>Figure 2</strong>  Signal pathway for transduction of umami taste sensationInosine monophosphate (IMP) has been suggested as enhancing (potentiating) the response of T1R1+T1R3 GPCRs to umami tastant molecules.  Scientists tested this hypothesis by culturing receptors from human embryonic kidney (HEK) cells containing promiscuous G proteins.  The HEK cells were then stimulated with umami tastants in the presence or absence of IMP.  In each test, the cells were exposed to the same amounts of amino acid tastant and IMP.  The number of cells that responded to each tastant was measured using a calcium indicator dye (FURA-2AM) in conjunction with fluorescence microscopy.  By comparing the cellular response ratio of the stimulated to unstimulated HEK cells, the extent of potentiation can be assessed.  The results are shown in Figure 3. <strong>Figure 3</strong>  Quantification of amino-acid responses for T1R1+T1R3 with and without IMP Adapted from Nelson G, Chandrashekar J, Hoon MA, et al. An amino-acid taste receptor. Nature. 2002;416(6877) :199-202. -Which of the following statements is true concerning the results shown in Figure 3? A) For the compounds potentiated by IMP, the extent of potentiation is five-fold. B) For the compounds potentiated by IMP, the extent of potentiation is equal. C) IMP most effectively potentiates Thr. D) IMP most effectively potentiates Asp. Figure 3  Quantification of amino-acid responses for T1R1+T1R3 with and without IMP
Adapted from Nelson G, Chandrashekar J, Hoon MA, et al. An amino-acid taste receptor. Nature. 2002;416(6877) :199-202.
-Which of the following statements is true concerning the results shown in Figure 3?


A) For the compounds potentiated by IMP, the extent of potentiation is five-fold.
B) For the compounds potentiated by IMP, the extent of potentiation is equal.
C) IMP most effectively potentiates Thr.
D) IMP most effectively potentiates Asp.

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