Under What Circumstance Would Applications of the Lineage Species Concept

Under what circumstance would applications of the lineage species concept most face limitations?

A) When studying asexual species of plants
B) When specimens have been collected under different environmental conditions
C) When determining the extent of divergence in DNA sequences necessary to determine whether different populations of salamanders should be considered one species or not
D) When determining the extent of reproductive isolation necessary to determine whether different populations of flies should be considered species.
E) When working with DNA collected from museum specimens of birds.

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