How Is Antibody Titer Determined

How is antibody titer determined?

A) Calculating the ratio of antibody to antigen in the highest possible sample dilution in which all antigen is bound
B) Measuring highest possible dilution where antibody is able to bind antigen
C) Measuring the minimum possible concentration of antibody necessary to bind all antigen present in a sample
D) Taking the reciprocal of the highest sample dilution ratio at which antibody is detectable

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