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A Piece of DNA Has Been Sequenced by Automated Dideoxy

Question 85

Multiple Choice

A piece of DNA has been sequenced by automated dideoxy sequencing, in which each dideoxyribonucleoside triphosphate was labeled with a fluorescent tag of a different color. The data corresponding to a small segment with the sequence 5′-TGCCACA-3′ is shown in the following diagram. Knowing that ddGTP has been labeled with green fluorescence, what color do you think was the fluorescent tag used to label ddTTP in the sequencing experiment? A piece of DNA has been sequenced by automated dideoxy sequencing, in which each dideoxyribonucleoside triphosphate was labeled with a fluorescent tag of a different color. The data corresponding to a small segment with the sequence 5′-TGCCACA-3′ is shown in the following diagram. Knowing that ddGTP has been labeled with green fluorescence, what color do you think was the fluorescent tag used to label ddTTP in the sequencing experiment? A) Blue B) Green C) Red D) Yellow


A) Blue
B) Green
C) Red
D) Yellow

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