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If Bacteria Transformed with a Recombinant PUC18 Plasmid Produce White

Question 4

Multiple Choice

If bacteria transformed with a recombinant pUC18 plasmid produce white colonies when grown on plates containing ampicillin and Xgal, which of the following is least likely?


A) They produce functional fi- galactosidase that cleaves X- gal.
B) They carry a vector with the bla gene used as the selectable marker.
C) They carry a vector with a lacZ gene that has been disrupted and rendered nonfunctional.
D) They carry a vector that contains a DNA fragment inserted into the multiple cloning site.
E) They produce fi- lactamase that provides resistance to ampicillin.

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